| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Department of Biological Sciences, University of Idaho, Moscow, Idaho 83844
b Department of Molecular and Integrative Physiology, Ralph L. Smith Research Center, University of Kansas Medical Center, Kansas City, Kansas 66103
Mutation of the leukemia inhibitory factor (LIF) gene results in reproductive failure in LIF -/- mice due to an inability to implant their blastocysts. This condition is reversed by infusion of LIF or by transferral of embryos to pseudopregnant, wild-type mice. This led us to hypothesize that embryonic diapause in the spotted skunk is due to insufficient uterine expression of LIF whereas resumption of development and implantation are associated with increased LIF expression. We also investigated the hormonal control of LIF expression. Uterine concentrations of LIF mRNA were determined by quantitative reverse transcription-polymerase chain reaction. Changes in cell-specific localization of LIF mRNA and protein were determined by in situ hybridization and immunocytochemistry. LIF mRNA was detected but was not abundant during embryonic diapause; it then increased when blastocysts resumed development and remained elevated prior to implantation. LIF mRNA and protein could not be localized in the uterus during embryonic diapause but were quite apparent in luminal and glandular epithelium during blastocyst activation. Prolactin, progesterone, and estradiol failed to increase uterine concentrations of LIF mRNA above those in ovariectomized controls. These data are consistent with the initial hypothesis and suggest that LIF may somehow be involved in preparing the uterus for implantation in the spotted skunk.
2 Correspondence. FAX: 208 885 7905; rmead{at}uidaho.edu
This article has been cited by other articles:
|
|
 |
|
  A.A Fouladi-Nashta, L Mohamet, J.K Heath, and S.J Kimber Interleukin 1 Signaling Is Regulated by Leukemia Inhibitory Factor (LIF) and Is Aberrant in Lif-/- Mouse Uterus Biol Reprod, July 1, 2008; 79(1): 142 - 153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Song and H. Lim Evidence for heterodimeric association of leukemia inhibitory factor (LIF) receptor and gp130 in the mouse uterus for LIF signaling during blastocyst implantation Reproduction, February 1, 2006; 131(2): 341 - 349. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. L Lopes, J. A Desmarais, and B. D Murphy Embryonic diapause and its regulation Reproduction, December 1, 2004; 128(6): 669 - 678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Daikoku, H. Song, Y. Guo, A. Riesewijk, S. Mosselman, S. K. Das, and S. K. Dey Uterine Msx-1 and Wnt4 Signaling Becomes Aberrant in Mice with the Loss of Leukemia Inhibitory Factor or Hoxa-10: Evidence for a Novel Cytokine-Homeobox-Wnt Signaling in Implantation Mol. Endocrinol., May 1, 2004; 18(5): 1238 - 1250. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Baran, P. A. Kelly, and N. Binart Decysin, a New Member of the Metalloproteinase Family, Is Regulated by Prolactin and Steroids During Mouse Pregnancy Biol Reprod, May 1, 2003; 68(5): 1787 - 1792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sato, T. Tsubota, T. Komatsu, G. Watanabe, K. Taya, T. Murase, I. Kita, and T. Kudo Changes in Sex Steroids, Gonadotropins, Prolactin, and Inhibin in Pregnant and Nonpregnant Japanese Black Bears (Ursus thibetanus japonicus) Biol Reprod, October 1, 2001; 65(4): 1006 - 1013. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-G. Cheng, J. R. Chen, L. Hernandez, W. G. Alvord, and C. L. Stewart Dual control of LIF expression and LIF receptor function regulate Stat3 activation at the onset of uterine receptivity and embryo implantation PNAS, June 28, 2001; (2001) 151180898. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Chen, J.-G. Cheng, T. Shatzer, L. Sewell, L. Hernandez, and C. L. Stewart Leukemia Inhibitory Factor Can Substitute for Nidatory Estrogen and Is Essential to Inducing a Receptive Uterus for Implantation But Is Not Essential for Subsequent Embryogenesis Endocrinology, December 1, 2000; 141(12): 4365 - 4372. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Song, H. Lim, S. K. Das, B. C. Paria, and S. K. Dey Dysregulation of EGF Family of Growth Factors and COX-2 in the Uterus during the Preattachment and Attachment Reactions of the Blastocyst with the Luminal Epithelium Correlates with Implantation Failure in LIF- Deficient Mice Mol. Endocrinol., August 1, 2000; 14(8): 1147 - 1161. [Abstract] [Full Text]
|
|
|
|
|
|
C. Passavant, X. Zhao, S.K. Das, S.K. Dey, and R.A. Mead Changes in Uterine Expression of Leukemia Inhibitory Factor Receptor Gene During Pregnancy and Its Up-Regulation by Prolactin in the Western Spotted Skunk Biol Reprod, July 1, 2000; 63(1): 301 - 307. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. J. Auernhammer and S. Melmed Leukemia-Inhibitory Factor--Neuroimmune Modulator of Endocrine Function Endocr. Rev., June 1, 2000; 21(3): 313 - 345. [Abstract] [Full Text]
|
|
|
|
 |
|
 K. C. Reinhart, R. K. Dubey, P. J. Keller, U. Lauper, and M. Rosselli Xeno-oestrogens and phyto-oestrogens induce the synthesis of leukaemia inhibitory factor by human and bovine oviduct cells Mol. Hum. Reprod., October 1, 1999; 5(10): 899 - 907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.K. Das, J. Wang, S.K. Dey, and R.A. Mead Spatiotemporal Expression of Cyclooxygenase 1 and Cyclooxygenase 2 during Delayed Implantation and the Periimplantation Period in the Western Spotted Skunk Biol Reprod, April 1, 1999; 60(4): 893 - 899. [Abstract] [Full Text]
|
|
|
|
|
|
J.-G. Cheng, J. R. Chen, L. Hernandez, W. G. Alvord, and C. L. Stewart Dual control of LIF expression and LIF receptor function regulate Stat3 activation at the onset of uterine receptivity and embryo implantation PNAS, July 17, 2001; 98(15): 8680 - 8685. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
