| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Center for Research of Endangered Wildlife, Cincinnati Zoo and Botanical Garden, Cincinnati, Ohio 45220
b Conservation & Research Center, National Zoological Park, Smithsonian Institution, Front Royal, Virginia 22630
A heterologous bovine in vitro fertilization (IVF) system was used to study the functional competence of scimitar-horned oryx spermatozoa after cryopreservation. Four sperm-freezing methods were compared after dilution of ejaculates from six oryx with an equine semen extender: 1) dry ice, 2) dry shipper one-step, 3) dry shipper two-step, and 4) liquid nitrogen vapor. Post-thaw sperm motility, longevity, and acrosomal status were assessed and zona pellucida penetration, fertilization, and embryo cleavage were evaluated after coincubation of thawed oryx spermatozoa with in vitro-matured domestic cow oocytes. Sperm motility index (SMI) decreased (p < 0.05) over a 6-h period, but a high percentage (
65%) of spermatozoa contained intact acrosomes in all treatments. Despite differences in sperm motility among methods, oocyte penetration, fertilization, and embryo cleavage did not differ (p 0.05). However, cleavage success was < 50% across all treatments. There were positive correlations (p < 0.05; r = 0.81–0.97) between sample SMI at 3 and 6 h and fertilization, penetration, and cleavage, but no correlations (p 0.05) between SMI at 0 or 1 h and IVF success. This study demonstrates that compatible heterologous gamete interaction allows thorough assessment of post-thaw sperm function in an endangered antelope. Scimitar-horned oryx spermatozoa appear relatively tolerant of varied cryopreservation methods, and preserved samples exhibit adequate post-thaw function to warrant use for assisted reproduction.
2 Correspondence: Terri L. Roth, Center for Research of Endangered Wildlife, Cincinnati Zoo and Botanical Garden, 3400 Vine Street, Cincinnati, OH 45220. FAX: 513 569 8213; terri.roth{at}cincyzoo.org
This article has been cited by other articles:
|
|
 |
|
  J. R. Herrick, P. Bartels, and R. L. Krisher Postthaw Evaluation of In Vitro Function of Epididymal Spermatozoa from Four Species of Free-Ranging African Bovids Biol Reprod, September 1, 2004; 71(3): 948 - 958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.J. Garde, A.J. Soler, J. Cassinello, C. Crespo, A.F. Malo, G. Espeso, M. Gomendio, and E.R.S. Roldan Sperm Cryopreservation in Three Species of Endangered Gazelles (Gazella cuvieri, G. dama mhorr, and G. dorcas neglecta) Biol Reprod, August 1, 2003; 69(2): 602 - 611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Soler and J. J. Garde Relationship Between the Characteristics of Epididymal Red Deer Spermatozoa and Penetrability Into Zona-Free Hamster Ova J Androl, May 1, 2003; 24(3): 393 - 400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Kouba, M. W. Atkinson, A. R. Gandolf, and T. L. Roth Species-Specific Sperm-Egg Interaction Affects the Utility of a Heterologous Bovine In Vitro Fertilization System for Evaluating Antelope Sperm Biol Reprod, October 1, 2001; 65(4): 1246 - 1251. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. L. Woo, P. F. James, and J. B Lingrel Sperm Motility Is Dependent on a Unique Isoform of the Na,K-ATPase J. Biol. Chem., June 30, 2000; 275(27): 20693 - 20699. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
