HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Teitsma, C. A. Articles by Kah, O. Search for Related Content PubMed PubMed Citation Articles by Teitsma, C. A. Articles by Kah, O. Agricola Articles by Teitsma, C. A. Articles by Kah, O.

Glucocorticoid Receptor Immunoreactivity in Neurons and Pituitary Cells Implicated in Reproductive Functions in Rainbow Trout: A Double Immunohistochemical Study¹

^a Endocrinologie Moléculaire de la Reproduction, UPRES-A CNRS 6026, Institut rennais d'Ecologie et Biologie des Poissons, Campus de Beaulieu, 35042 Rennes cedex, France

In order to identify the nature of the glucocorticoid receptor (GR)-expressing neurons and pituitary cells that potentially mediate the negative effects of stress on reproductive performance, double immunohistochemical stainings were performed in the brain and pituitary of the rainbow trout (Oncorhynchus mykiss). To avoid possible cross-reactions during the double staining studies, combinations of primary antibodies raised in different species were used, and we report here the generation of an antibody raised in guinea pig against the rainbow trout glucocorticoid receptor (rtGR). The results obtained in vitellogenic females showed that GnRH-positive neurons in the caudal telencephalon/anterior preoptic region consistently exhibited rtGR immunoreactivity. Similarly, in the anterior ventral preoptic region, a group of tyrosine hydroxylase-positive neurons, known for inhibiting gonadotropin (GTH)-2 secretion during vitellogenesis, was consistently shown to strongly express GR. Finally, we show that a large majority of the GTH-1 (FSH-like) and GTH-2 (LH-like) cells of the pituitary exhibit rtGR immunoreactivity. These results indicate that cortisol may affect the neuroendocrine control of the reproductive process of the rainbow trout at multiple sites.

¹ Supported by the European Union (FAIR GT 95–2549), the CNRS, the INRA, and the Fondation Langlois.

² Correspondence. FAX: 33 2 99 28 67 94; olivier.kah{at}univ-rennes1.fr

This article has been cited by other articles:

				T. Kitahashi, S. Ogawa, T. Soga, Y. Sakuma, and I. Parhar Sexual Maturation Modulates Expression of Nuclear Receptor Types in Laser-Captured Single Cells of the Cichlid (Oreochromis niloticus) Pituitary Endocrinology, December 1, 2007; 148(12): 5822 - 5830. [Abstract] [Full Text] [PDF]

				F.-A. Weltzien, C. Pasqualini, M.-E. Sebert, B. Vidal, N. Le Belle, O. Kah, P. Vernier, and S. Dufour Androgen-Dependent Stimulation of Brain Dopaminergic Systems in the Female European Eel (Anguilla anguilla) Endocrinology, June 1, 2006; 147(6): 2964 - 2973. [Abstract] [Full Text] [PDF]

				L. L. Carruth, R. E. Jones, and D. O. Norris Cortisol and Pacific Salmon: A New Look at the Role of Stress Hormones in Olfaction and Home-stream Migration Integr. Comp. Biol., July 1, 2002; 42(3): 574 - 581. [Abstract] [Full Text] [PDF]

				D. Consten, J. Bogerd, J. Komen, J.G.D. Lambert, and H.J.Th. Goos Long-Term Cortisol Treatment Inhibits Pubertal Development in Male Common Carp, Cyprinus carpio L Biol Reprod, April 1, 2001; 64(4): 1063 - 1071. [Abstract] [Full Text]