| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan 48201
Ovarian hormone secretion is regulated by gonadotropins, and it has been demonstrated that this response is modulated by nitric oxide (NO). The focus of this study was to determine the effect of chronic NO deficiency on the secretion of ovarian steroids. Female rats were given N-nitro-L-arginine (L-NNA; 0.6 g/L) in their drinking water, and vaginal smears were obtained daily. By 4 wk of treatment, all the rats were in constant estrus or proestrus. At 6–8 wk the animals were killed; the ovaries were removed and incubated in the presence of eCG (1 IU/ml) and hCG (1 IU/ml) and/or S-nitroso-L-acetyl penicillamine (an NO donor, S-NAP; 0.1 mM) for 4 h. Medium was collected at 30-min intervals, and estradiol, progesterone, and androstenedione were measured. Ovaries from proestrous rats served as controls. Ovaries from L-NNA-treated animals had a greater basal and gonadotropin-stimulated release of estradiol but not of androstenedione or progesterone in comparison to ovaries from untreated controls. S-NAP decreased the gonadotropin-stimulated estradiol, progesterone, and androstenedione in ovaries from NO-deficient rats. Steroid secretion in controls was not responsive to S-NAP. We conclude that chronic NO inhibition produces constant estrus due to increased estradiol production and that NO acts to inhibit estradiol and androstenedione production.
2 Correspondence: Joseph C. Dunbar, Department of Physiology, Wayne State University School of Medicine, 540 E. Canfield, Detroit, MI 48201. FAX: 313 577 5494; jdunbar{at}med.wayne.edu
This article has been cited by other articles:
|
|
 |
|
  V Tripathi and A Krishna Changes in nitric oxide (NO) synthase isoforms and NO in the ovary of Heteropneustes fossilis (Bloch.) during the reproductive cycle J. Endocrinol., November 1, 2008; 199(2): 307 - 316. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. V. Orozco, Z. Sosa, V. Fillipa, F. Mohamed, and A. M. Rastrilla The cholinergic influence on the mesenteric ganglion affects the liberation of ovarian steroids and nitric oxide in oestrus day rats: characterization of an ex vivo system J. Endocrinol., December 1, 2006; 191(3): 587 - 598. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Tamanini, G. Basini, F. Grasselli, and M. Tirelli Nitric oxide and the ovary J Anim Sci, February 1, 2003; 81(14_suppl_2): E1 - 7. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. C. Alexandreanu and D. M. Lawson Heme Oxygenase in the Rat Ovary: Immunohistochemical Localization and Possible Role in Steroidogenesis Experimental Biology and Medicine, January 1, 2003; 228(1): 59 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Barnes, K. Lapanowski, J. A. Rafols, D. M. Lawson, and J. C. Dunbar Chronic Nitric Oxide Deficiency Is Associated with Altered Leutinizing Hormone and Follicle-Stimulating Hormone Release in Ovariectomized Rats Experimental Biology and Medicine, October 1, 2002; 227(9): 817 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Barnes, K. Lapanowski, J. A. Rafols, D. M. Lawson, and J. C. Dunbar GnRH and Gonadotropin Release Is Decreased in Chronic Nitric Oxide Deficiency Experimental Biology and Medicine, July 1, 2001; 226(7): 701 - 706. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
