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Expression and Localization of Messenger Ribonucleic Acid for the Vitellogenin Receptor in Ovarian Follicles Throughout Oogenesis in the Rainbow Trout, Oncorhynchus mykiss¹

^a Laboratoire de Biologie de la Reproduction des Poissons, Unité Associée INRA, Université Bordeaux I, 33405 Talence, France ^b Department of Biology and Biochemistry, Brunel University, Uxbridge, Middlesex, UB8 3PH, United Kingdom ^c Laboratoire d'Histologie-Embryologie, Université Bordeaux II, 33076 Bordeaux, France ^d Laboratoire d'Endocrinologie Moleculaire de la Reproduction, UPRES-A 6026, CNRS, Université de Rennes I, 35042 Rennes, France ^e Department of Molecular Genetics, Biocenter and University of Vienna, A-1030, Vienna, Austria

The expression and localization of vitellogenin (VTG) receptor (VTGR) mRNA were identified throughout ovarian development in the rainbow trout, Oncorhynchus mykiss. Northern blot confirmed the presence of a transcript (approximately 3.9 kilobases [kb]) that was specific to the ovary. The expression of VTGR mRNA varied throughout ovarian development and was highest in previtellogenic ovaries and in ovaries at the onset of vitellogenesis containing ovarian follicles (OF) from 35 to 600 µm in diameter. In situ hybridization using ³⁵S riboprobes showed that the transcription of the VTGR gene was initiated in OF measuring 45–50 µm in diameter, with transcripts being exclusively localized in the ooplasm. A dramatic increase in mRNA synthesis occurred during previtellogenic growth (OF from 50 to 200 µm); this was followed by a gradual decrease during the vitellogenic growth phase. VTGR mRNA was not detected in OF greater than 1000 µm in diameter (oocytes actively sequestering VTG). Immunocytolocalization of yolk proteins derived from VTG demonstrated that oocytes started to sequester VTG when they were around 300 µm in diameter, shortly after the time of maximal density of VTGR mRNA in the ooplasm. The timing of transcription of the VTGR gene, predominantly during previtellogenesis, suggests that the VTGR is recycled to the oocyte surface during the vitellogenic growth phase.

¹ This work was supported by grants from the FEDER 5b with the participation of the fish farm Les Viviers de France. L.M.P. had a scholarship from the Brazilian National Research Council (CNPq). K.C. was funded by a BBSRC (so 1942) grant to C.R.T.

² Correspondence: F. Le Menn: Laboratoire de Biologie de la Reproduction des Poissons, Unité Associée INRA, Université Bordeaux I, Avenue des Facultés, 33405 Talence, France. FAX: 33 5 56 84 89 15; lemenn{at}ubrpinra.u-bordeaux.fr

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