Biol Reprod Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Payne, S. G.
Right arrow Articles by Guilbert, L. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Payne, S. G.
Right arrow Articles by Guilbert, L. J.
Agricola
Right arrow Articles by Payne, S. G.
Right arrow Articles by Guilbert, L. J.
Biology of Reproduction 60, 1144-1150 (1999)
©Copyright 1999 Society for the Study of Reproduction, Inc.

Death Receptor Fas/Apo-1/CD95 Expressed by Human Placental Cytotrophoblasts Does Not Mediate Apoptosis1

Shawn G. Payne3,a, Steve C. Smith3,d, Sandra T. Davidgeb,c, Phillip N. Bakerd, and Larry J. Guilbert2,a,c

a Departments of Medical Microbiology and Immunology and b Obstetrics and Gynecology, c The University of Alberta Perinatal Research Centre, University of Alberta, Edmonton, Alberta, Canada T6G 2H7 d Department of Obstetrics, City Hospital, University of Nottingham, Nottingham, NG7 2UH, United Kingdom

Trophoblasts, the fetal cells that line the villous placenta and separate maternal blood from fetal tissue, express both Fas antigen and the tumor necrosis factor (TNF) receptor p55 (TNFRp55), two members of the TNF receptor family that contain a cytoplasmic "death domain" that mediates apoptotic signals. We show that Fas mRNA expressed by cultured villous cytotrophoblasts isolated from term placentas encodes transmembrane sequences and that the protein is full-length (approximately 45 kDa), suggesting that the product is an active plasma membrane-anchored receptor. Its location on the cell surface was confirmed by cellular ELISA analysis of live cells. Although cytotrophoblast apoptosis was induced by TNF{alpha}, and both anti-Fas antibody (CH11) and FasL-expressing T lymphocyte hybridoma (activated A1.1) cells induced HeLa cell apoptosis, neither CH11 antibody nor activated A1.1 cells stimulated apoptosis in term or first-trimester cytotrophoblasts or in term syncytiotrophoblasts. We conclude that Fas- but not TNFRp55-mediated apoptosis is blocked in primary villous trophoblasts. These data suggest that the Fas response is specifically inactivated by unknown mechanisms to avoid autocrine or paracrine killing by Fas ligand constitutively expressed on neighboring cyto- or syncytiotrophoblasts.

1 Supported by grants from the Toronto Sick Children's Hospital Foundation (L.J.G.), the University of Alberta Hospital Foundation (L.J.G.), and an interlaboratory collaboration grant from the Wellcome Trust (P.N.B. and S.T.D.). S.P. is supported by an Alberta Heritage for Medical Research Studentship, and S.S. is a Wellbeing training fellow.

2 Correspondence: Larry J. Guilbert, Dept. Medical Microbiology and Immunology, 1–41 Medical Sciences Building, University of Alberta, Edmonton, AB, Canada T6G 2H7. FAX: 403 492 9828; larry.guilbert{at}ualberta.ca

3 S. Payne and S. Smith contributed equally to this publication and are co-first authors.




This article has been cited by other articles:


Home page
 Mol Hum ReprodHome page
V. Minas, U. Jeschke, S.N. Kalantaridou, D.U. Richter, T. Reimer, I. Mylonas, K. Friese, and A. Makrigiannakis
Abortion is associated with increased expression of FasL in decidual leukocytes and apoptosis of extravillous trophoblasts: a role for CRH and urocortin
Mol. Hum. Reprod., September 1, 2007; 13(9): 663 - 673.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. L. Straszewski-Chavez, I. P. Visintin, N. Karassina, G. Los, P. Liston, R. Halaban, A. Fadiel, and G. Mor
XAF1 Mediates Tumor Necrosis Factor-{alpha}-induced Apoptosis and X-linked Inhibitor of Apoptosis Cleavage by Acting through the Mitochondrial Pathway
J. Biol. Chem., April 27, 2007; 282(17): 13059 - 13072.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
S. L. Straszewski-Chavez, V. M. Abrahams, and G. Mor
The Role of Apoptosis in the Regulation of Trophoblast Survival and Differentiation during Pregnancy
Endocr. Rev., December 1, 2005; 26(7): 877 - 897.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F. J. Rubio Pomar, B. A.J. Roelen, K. A. Slot, H. T.A. van Tol, B. Colenbrander, and K. J. Teerds
Role of Fas-Mediated Apoptosis and Follicle-Stimulating Hormone on the Developmental Capacity of Bovine Cumulus Oocyte Complexes In Vitro
Biol Reprod, September 1, 2004; 71(3): 790 - 796.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
R. M. Gill and J. S. Hunt
Soluble Receptor (DcR3) and Cellular Inhibitor of Apoptosis-2 (cIAP-2) Protect Human Cytotrophoblast Cells Against LIGHT-Mediated Apoptosis
Am. J. Pathol., July 1, 2004; 165(1): 309 - 317.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
M. A. Turner, S. Vause, and S. L. Greenwood
The Regulation of Interleukin-6 Secretion by Prostanoids and Members of the Tumor Necrosis Factor Superfamily in Fresh Villous Fragments of Term Human Placenta
Reproductive Sciences, April 1, 2004; 11(3): 141 - 148.
[Abstract] [PDF]

Home page
 Mol Hum ReprodHome page
S. L. Straszewski-Chavez, V. M. Abrahams, E. F. Funai, and G. Mor
X-linked inhibitor of apoptosis (XIAP) confers human trophoblast cell resistance to Fas-mediated apoptosis
Mol. Hum. Reprod., January 1, 2004; 10(1): 33 - 41.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. Lonergan, D. Aponso, K. W. Marvin, R. J. A. Helliwell, T. A. Sato, M. D. Mitchell, T. Chaiwaropongsa, R. Romero, and J. A. Keelan
Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL), TRAIL Receptors, and the Soluble Receptor Osteoprotegerin in Human Gestational Membranes and Amniotic Fluid during Pregnancy and Labor at Term and Preterm
J. Clin. Endocrinol. Metab., August 1, 2003; 88(8): 3835 - 3844.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D. R Balkundi, J. A Ziegler, J. F Watchko, C. Craven, and M. Trucco
Regulation of FasL/Fas in Human Trophoblasts: Possible Implications for Chorioamnionitis
Biol Reprod, August 1, 2003; 69(2): 718 - 724.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
G. Chan, D. G. Hemmings, A. D. Yurochko, and L. J. Guilbert
Human Cytomegalovirus-Caused Damage to Placental Trophoblasts Mediated by Immediate-Early Gene-Induced Tumor Necrosis Factor-{alpha}
Am. J. Pathol., October 1, 2002; 161(4): 1371 - 1381.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. Aschkenazi, S. Straszewski, K. M.A. Verwer, H. Foellmer, T. Rutherford, and G. Mor
Differential Regulation and Function of the Fas/Fas Ligand System in Human Trophoblast Cells
Biol Reprod, June 1, 2002; 66(6): 1853 - 1861.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
N. Di Simone, R. Castellani, D. Caliandro, and A. Caruso
Monoclonal Anti-Annexin V Antibody Inhibits Trophoblast Gonadotropin Secretion and Induces Syncytiotrophoblast Apoptosis
Biol Reprod, December 1, 2001; 65(6): 1766 - 1770.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. Gruslin, Q. Qiu, and B. K. Tsang
Influence of Maternal Smoking on Trophoblast Apoptosis Throughout Development: Possible Involvement of Xiap Regulation
Biol Reprod, October 1, 2001; 65(4): 1164 - 1169.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. Gruslin, Q. Qiu, and B. K. Tsang
X-Linked Inhibitor of Apoptosis Protein Expression and the Regulation of Apoptosis During Human Placental Development
Biol Reprod, April 1, 2001; 64(4): 1264 - 1272.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
S. Wongweragiat, R. F. Searle, and J. N. Bulmer
Expression of Fas/Fas Ligand by Decidual Leukocytes in Hydatidiform Mole
Biol Reprod, March 1, 2001; 64(3): 784 - 789.
[Abstract] [Full Text]

Home page
 J. Leukoc. Biol.Home page
M. I. Garcia-Lloret, B. Winkler-Lowen, and L. J. Guilbert
Monocytes adhering by LFA-1 to placental syncytiotrophoblasts induce local apoptosis via release of TNF-{alpha}. A model for hematogenous initiation of placental inflammations
J. Leukoc. Biol., December 1, 2000; 68(6): 903 - 908.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the Society for the Study of Reproduction.