HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hamano, K.-i. Articles by Minato, Y. Search for Related Content PubMed PubMed Citation Articles by Hamano, K.-i. Articles by Minato, Y. Agricola Articles by Hamano, K.-i. Articles by Minato, Y.

Gender Preselection in Cattle with Intracytoplasmically Injected, Flow Cytometrically Sorted Sperm Heads¹

^a Maebashi Institute of Animal Science, Livestock Improvement Association of Japan, Inc., Maebashi, Gunma, 371–0121, Japan

We investigated the development to the blastocyst and subsequent live-offspring stages of in vitro-matured bovine oocytes intracytoplasmically injected with flow cytometrically sorted bull sperm heads. Bull sperm heads, prepared by ultrasound sonication, were distinguished and sorted on the basis of their relative DNA contents using a flow cytometer/cell sorter modified for sorting sperm. By fluorescence in situ hybridization, the proportion of sperm confirmed as having Y specific DNA in the fraction sorted for the Y sperm was 82%. Injection with single sorted sperm heads of in vitro-matured oocytes (cultured for 24 h) resulted in 46.6% cleavage and 6.9% blastocyst development rates. Embryo transfer of 48 blastocysts (Days 7–8) to recipients (one per recipient) resulted in 20.8% pregnancy and 20.8% normal live offspring production rates. The birth of 8 male and 2 female calves represents an 80% sex preselection accuracy rate.

¹ This work was supported by a grant from the Agriculture and Livestock Industries Corporation and Ministry of Agriculture, Forestry and Fisheries.

² Correspondence: Yoshiaki Minato, Maebashi Institute of Animal Science, Livestock Improvement Association of Japan, Inc., 316 Kanamarumachi, Maebashi, Gunma, 371–0121, Japan. FAX: 81 27 269 9526; res-liaj{at}tohgoku.or.up

³ Current address: Research Farm, Faculty of Agriculture, Shinshu University, 8304 Minamiminowa, Kamiina, Nagano 399–4598, Japan.

This article has been cited by other articles:

				J.-L. Liu, H. Kusakabe, C.-C. Chang, H. Suzuki, D. W. Schmidt, M. Julian, R. Pfeffer, C. L. Bormann, X. C. Tian, R. Yanagimachi, et al. Freeze-Dried Sperm Fertilization Leads to Full-Term Development in Rabbits Biol Reprod, June 1, 2004; 70(6): 1776 - 1781. [Abstract] [Full Text] [PDF]

				J.-W. Lee, X. C. Tian, and X. Yang Failure of Male Pronucleus Formation Is the Major Cause of Lack of Fertilization and Embryo Development in Pig Oocytes Subjected to Intracytoplasmic Sperm Injection Biol Reprod, April 1, 2003; 68(4): 1341 - 1347. [Abstract] [Full Text] [PDF]

				M. Nakai, N. Kashiwazaski, A. Takizawa, Y. Hayashi, E. Nakatsukasa, D.-i. Fuchimoto, J. Noguchi, H. Kaneko, M. Shino, and K. Kikuchi Viable Piglets Generated from Porcine Oocytes Matured In Vitro and Fertilized by Intracytoplasmic Sperm Head Injection Biol Reprod, March 1, 2003; 68(3): 1003 - 1008. [Abstract] [Full Text] [PDF]

				J. Wu, D. T. Carrell, and A. L. Wilcox Development of In Vitro-Matured Oocytes from Porcine Preantral Follicles Following Intracytoplasmic Sperm Injection Biol Reprod, November 1, 2001; 65(5): 1579 - 1585. [Abstract] [Full Text] [PDF]

				M. J. Martin Development of In Vivo-Matured Porcine Oocytes Following Intracytoplasmic Sperm Injection Biol Reprod, July 1, 2000; 63(1): 109 - 112. [Abstract] [Full Text]