| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a Department of Genetics, Southwest Foundation for Biomedical Research, San Antonio, Texas 78245
b Department of Cellular & Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, Texas 78284
The PGK2 gene is expressed in a strictly tissue-specific manner in meiotic spermatocytes and postmeiotic spermatids during spermatogenesis in eutherian mammals. Previous results indicate that this is regulated at the transcriptional level by core promoter sequences that bind ubiquitous transcription factors and by sequences in a 40-base pair (bp) upstream enhancer region (E1/E4) that bind tissue-specific transcription factors. Transgenic mice carrying different PGK2 promoter sequences linked to the chloramphenicol acetyltransferase (CAT) reporter gene, one containing only the 40-bp E1/E4 enhancer sequence plus the core promoter and two containing 515 bp of PGK2 promoter but with either the E1/E4 enhancer region or the Sp1-binding site in the core promoter disrupted by in vitro mutagenesis, all showed levels of expression reduced to less than half that of the wild-type 515 PGK2/CAT transgene. These results indicate that multiple factor-binding regions normally regulate initiation of transcription from the PGK2 promoter. The single disruption of any one of these binding activities reduced, but did not abolish, transgene expression. This is consistent with an "enhanceosome"-like function in this promoter involving multiple bound activator proteins that interact in a combinatorial manner to synergistically promote testis-specific transcription.
2 Correspondence: John R. McCarrey, Southwest Foundation for Biomedical Research, P.O. Box 760549, San Antonio, TX 78245. FAX: 210 670 3316; johnmc{at}darwin.sfbr.org
This article has been cited by other articles:
|
|
 |
|
  H. Yoshioka, C. B. Geyer, J. L. Hornecker, K. T. Patel, and J. R. McCarrey In Vivo Analysis of Developmentally and Evolutionarily Dynamic Protein-DNA Interactions Regulating Transcription of the Pgk2 Gene during Mammalian Spermatogenesis Mol. Cell. Biol., November 15, 2007; 27(22): 7871 - 7885. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Dan, Y. Lifang, and L. Guangxiu Expression and Possible Functions of a Novel Gene SPATA12 in Human Testis J Androl, July 1, 2007; 28(4): 502 - 512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Xu and N. B. Hecht Polypyrimidine Tract Binding Protein 2 Stabilizes Phosphoglycerate Kinase 2 mRNA in Murine Male Germ Cells by Binding to Its 3'UTR Biol Reprod, June 1, 2007; 76(6): 1025 - 1033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kim, D. Li, Y. Cui, K. Mueller, W. C. Chears, and J. DeJong Regulatory Factor Interactions and Somatic Silencing of the Germ Cell-specific ALF Gene J. Biol. Chem., November 10, 2006; 281(45): 34288 - 34298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Rohozinski, D. J. Lamb, and C. E. Bishop UTP14c Is a Recently Acquired Retrogene Associated with Spermatogenesis and Fertility in Man Biol Reprod, April 1, 2006; 74(4): 644 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Liu, N. Pore, M. Kim, K. R. Voong, M. Dowling, A. Maity, and G. D. Kao Regulation of Histone Deacetylase 4 Expression by the SP Family of Transcription Factors Mol. Biol. Cell, February 1, 2006; 17(2): 585 - 597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Alimov, O.-K. Park-Sarge, K. D. Sarge, H. H. Malluche, and N. J. Koszewski Transactivation of the Parathyroid Hormone Promoter by Specificity Proteins and the Nuclear Factor Y Complex Endocrinology, August 1, 2005; 146(8): 3409 - 3416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, J. T. San Agustin, G. B. Witman, and D. L. Kilpatrick Novel Role for a Sterol Response Element Binding Protein in Directing Spermatogenic Cell-Specific Gene Expression Mol. Cell. Biol., December 15, 2004; 24(24): 10681 - 10688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Han, W. Xie, S. H. Kim, L. Yue, and J. DeJong A Short Core Promoter Drives Expression of the ALF Transcription Factor in Reproductive Tissues of Male and Female Mice Biol Reprod, September 1, 2004; 71(3): 933 - 941. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Sha, Z. Zhou, J. Li, L. Yin, H. Yang, G. Hu, M. Luo, H. C. Chan, and K. Zhou Identification of testis development and spermatogenesis-related genes in human and mouse testes using cDNA arrays Mol. Hum. Reprod., June 1, 2002; 8(6): 511 - 517. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
