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a School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6009
Recently a system was developed in which transplanted donor spermatogonial stem cells establish complete spermatogenesis in the testes of an infertile recipient. To obtain insight into stem cell activity and the behavior of donor germ cells, the pattern and kinetics of mouse spermatogonial colonization in recipient seminiferous tubules were analyzed during the 4 mo following transplantation. The colonization process can be divided into three continuous phases. First, during the initial week, transplanted cells were randomly distributed throughout the tubules, and a small number reached the basement membrane. Second, from 1 wk to 1 mo, donor cells on the basement membrane divided and formed a monolayer network. Third, beginning at about 1 mo and continuing throughout the observation period, cells in the center of the network differentiated extensively and established a colony of spermatogenesis, which expanded laterally by repeating phase two and then three. An average of 19 donor cell-derived colonies developed from 106 cells transplanted to the seminiferous tubules of a recipient testis; the number of colonized sites did not change between 1 and 4 mo. However, the length of the colonies increased from 0.73 to 5.78 mm between 1 and 4 mo. These experiments establish the feasibility of studying in a systematic and quantitative manner the pattern and kinetics of the colonization process. Using spermatogonial transplantation as a functional assay, it should be possible to assess the effects of various treatments on stem cells and on recipient seminiferous tubules to provide unique insight into the process of spermatogenesis.
2 Correspondence: R.L. Brinster, School of Veterinary Medicine, 3850 Baltimore Avenue, Philadelphia, PA 19104–6009. FAX: 215 898–0667.
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M. Nagano, C. J. Brinster, K. E. Orwig, B.-Y. Ryu, M. R. Avarbock, and R. L. Brinster Transgenic mice produced by retroviral transduction of male germ-line stem cells PNAS, October 16, 2001; (2001) 231473498. [Abstract] [Full Text] [PDF]
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H. Chiarini-Garcia, J. R. Hornick, M. D. Griswold, and L. D. Russell Distribution of Type A Spermatogonia in the Mouse Is Not Random Biol Reprod, October 1, 2001; 65(4): 1179 - 1185. [Abstract] [Full Text] [PDF]
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T. Shinohara, K. E. Orwig, M. R. Avarbock, and R. L. Brinster From the Cover: Remodeling of the postnatal mouse testis is accompanied by dramatic changes in stem cell number and niche accessibility PNAS, May 22, 2001; 98(11): 6186 - 6191. [Abstract] [Full Text] [PDF]
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T. Shinohara, K. E. Orwig, M. R. Avarbock, and R. L. Brinster From the Cover: Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells PNAS, July 18, 2000; 97(15): 8346 - 8351. [Abstract] [Full Text] [PDF]
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I. Dobrinski, M. R. Avarbock, and R. L. Brinster Transplantation of Germ Cells from Rabbits and Dogs Into Mouse Testes Biol Reprod, November 1, 1999; 61(5): 1331 - 1339. [Abstract] [Full Text]
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M. Nagano, C. J. Brinster, K. E. Orwig, B.-Y. Ryu, M. R. Avarbock, and R. L. Brinster From the Cover: Transgenic mice produced by retroviral transduction of male germ-line stem cells PNAS, November 6, 2001; 98(23): 13090 - 13095. [Abstract] [Full Text] [PDF]
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