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Identification and Expression of G_o1 and G_o2 -Subunit Transcripts in Human Myometrium in Relation to Pregnancy

^a INSERM U. 361, Université René Descartes Paris V, Pavillon Baudelocque, 75014 Paris, France

The 39-kDa G_o protein, the subunit of a major heterotrimeric G protein of brain and neuroendocrine cells, was found to be present in human myometrium. Using three different antisera, we showed its strong expression in myometrium from pregnant patients as compared to nonpregnant ones. This is in agreement with the high expression level of its two isoforms (_o1 and _o2), previously described in late pregnancy. To better ascertain the nature of these immunoreactive isoforms, we investigated transcripts of the G_o gene in myometrium from pregnant and nonpregnant patients by reverse transcription-polymerase chain reaction (RT-PCR). In this tissue, the amplified cDNA product of a region common to both G_o1 and G_o2 mRNA variants was recognized as the G_o nucleotide sequence. Transcripts of G_o1 and G_o2 were identified by sequencing. A partial cDNA G_o2 sequence was described, which differed from the G_o gene by two nucleotides in exon 8B. Levels of G_o1 and G_o2 transcripts analyzed by semi-quantitative RT-PCR were significantly higher in myometrium from pregnant than from nonpregnant patients. It is suggested that G_o gene expression in this tissue may contribute to modifications seen in the signaling pathways observed at the end of pregnancy.

¹ Correspondence: Paulette Duc-Goiran, U. 361, Pavillon Baudelocque, 123, Bld de Port-Royal, 75014 Paris, France. FAX: 331 43 26 44 08; u361{at}cochin.inserm.fr

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