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Hyperactivation of Hamster Sperm Motility by Temperature-Dependent Tyrosine Phosphorylation of an 80-kDa Protein¹

^a Department of Biology, College of Arts and Sciences, The University of Tokyo, Komaba, Meguro-ku, Tokyo 153, Japan

Protein phosphorylation and dephosphorylation are believed to play key roles in regulation of sperm motility. Here we examine the effect of temperature on hamster sperm motility and protein tyrosine phosphorylation status. As in previous work, a decrease from 37°C to 22°C caused loss of hyperactivated motility. We now find that cooling also produces a dephosphorylation of several 48–80-kDa flagellar peptides. A return to 37°C restored hyperactivation but resulted in rephosphorylation of only an 80-kDa protein. Conversely, hyperactivation and phosphorylation of the 80-kDa component were insensitive to incubation temperature for sperm incubated with the protein phosphatase inhibitor, calyculin A, or for sperm demembranated by detergent extraction. These results strongly indicate that the temperature-sensitive tyrosine phosphorylation status of an 80-kDa sperm flagellar peptide explains the sensitivity of hyperactivation to temperature.

¹ This work was supported by research grants P-95118 and 07–95118 from the Japan Society for the Promotion of Science.

² Correspondence: Yuming Si, Department of Anatomy and Cell Biology, Temple University School of Medicine, 3400 North Broad Street, Philadelphia, PA 19140. FAX: 215 707 2966; ysi{at}nimbus.temple.edu

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