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a Center for Recombinant Gamete Contraceptive Vaccinogens, University of Virginia Health Sciences Center, Charlottesville, Virginia 22908
b Department of Obstetrics and Gynecology, Hyogo Medical College, Hyogo, Japan
c Department of Obstetrics and Gynecology and Reproductive Medicine, Health Science Center, Stony Brook, New York 11794-8091
The objective of this study was to identify those immunodominant sperm antigens recognized by antisperm antibodies (ASA) in the serum samples of infertile men and women. High-resolution two-dimensional gel electrophoresis was employed to separate human sperm proteins using isoelectric focusing or nonequilibrium pH gradient electrophoresis, followed by PAGE. Serum samples from 15 infertile male subjects and 6 infertile female subjects that contained ASA as assayed by the immunobead binding test (IBT) were analyzed by Western blotting followed by enhanced chemiluminescence (ECL). Serum samples from 10 fertile subjects (5 males and 5 females) that were ASA negative by IBT were used as controls. The ECL blots were analyzed by computer scanning to compare the immunoreactivity between serum samples from fertile and infertile subjects and to identify the antigens unique to the sera of the infertile subjects; 98 sperm auto- and iso-antigenic protein spots were recognized by sera from infertile males and females but not from fertile subjects. Based on vectorial labeling with 125I at the sperm surface, a subset of 6 auto- and iso-antigens was identified as possibly relevant to antibody-mediated infertility.
2 Correspondence: John C. Herr, University of Virginia, Health Sciences Center, Box #439, Charlottesville, VA 22908. FAX: 804 982 3912; jch7k{at}virginia.edu
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