| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
Induces a Rapid Decline in Progesterone Production and Steroidogenic Acute Regulatory Protein Expression in Isolated Rat Corpus Luteum Without Altering Messenger Ribonucleic Acid Expression1
a Reproductive Endocrinology, Infertility and Genetics Section, Department of Obstetrics and Gynecology, The Medical College of Georgia, Augusta, Georgia 30912
b Department of Physiology and Biophysics, The University of Illinois at Chicago, Chicago, Illinois 60612
With interest in steroidogenic acute regulatory protein (StAR) involvement in the luteolytic process, we studied changes in serum progesterone levels and the concomitant expression of StAR mRNA and protein (37-, 32-, and 30-kDa forms) in postovulatory Day 7 corpora lutea (CL) isolated from rats 1 h after injection with prostaglandin F2
(PGF2, n = 6) or saline (n = 6). Serum progesterone levels were determined by RIA, StAR and ß-actin mRNA expression by Northern analysis, and StAR and ß-actin protein expression by Western analysis. Adrenal, brain, and spleen from control animals were used as positive and negative controls for StAR expression. Scanning optical densitometry measurements were standardized by dividing the signal strength from each StAR autoradiogram lane by that from the corresponding ß-actin autoradiogram lane. ANOVA was used for significance testing, with set at 0.05. The 37-, 32-, and 30-kDa forms of StAR protein were expressed in all adrenal samples, whereas only the 37- and 30-kDa forms were found in CL. Serum progesterone levels and expression of the 30-kDa and 37-kDa forms of the StAR protein in CL were all found to be significantly lower in the PGF2-treated than the saline-treated group. StAR mRNA expression was not significantly different in the saline- and PGF2-treated rats. The rapid decline in StAR protein expression that accompanies PGF2 induced luteolysis, therefore, does not result from significant decline in mRNA expression.
2 Correspondence and current address: Iqbal Khan, Department of Obstetrics and Gynecology, The Medical College of Georgia, Augusta, GA 30912. FAX: 706 721 6830; ikhan{at}mail.mcg.edu
This article has been cited by other articles:
|
|
 |
|
  Q. Liu, K. A. Merkler, X. Zhang, and M. P. McLean Prostaglandin F2{alpha} Suppresses Rat Steroidogenic Acute Regulatory Protein Expression via Induction of Yin Yang 1 Protein and Recruitment of Histone Deacetylase 1 Protein Endocrinology, November 1, 2007; 148(11): 5209 - 5219. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. C. Wathes, D. R. E. Abayasekara, and R. J. Aitken Polyunsaturated Fatty Acids in Male and Female Reproduction Biol Reprod, August 1, 2007; 77(2): 190 - 201. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. R Manna, Y. Jo, and D. M Stocco Regulation of Leydig cell steroidogenesis by extracellular signal-regulated kinase 1/2: role of protein kinase A and protein kinase C signaling J. Endocrinol., April 1, 2007; 193(1): 53 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Frungieri, S. I. Gonzalez-Calvar, F. Parborell, M. Albrecht, A. Mayerhofer, and R. S. Calandra Cyclooxygenase-2 and Prostaglandin F2{alpha} in Syrian Hamster Leydig Cells: Inhibitory Role on Luteinizing Hormone/Human Chorionic Gonadotropin-Stimulated Testosterone Production Endocrinology, September 1, 2006; 147(9): 4476 - 4485. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. R Manna, S. P Chandrala, Y. Jo, and D. M Stocco cAMP-independent signaling regulates steroidogenesis in mouse Leydig cells in the absence of StAR phosphorylation. J. Mol. Endocrinol., August 1, 2006; 37(1): 81 - 95. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. P. Piekorz, S. Gingras, A. Hoffmeyer, J. N. Ihle, and Y. Weinstein Regulation of Progesterone Levels during Pregnancy and Parturition by Signal Transducer and Activator of Transcription 5 and 20{alpha}-Hydroxysteroid Dehydrogenase Mol. Endocrinol., February 1, 2005; 19(2): 431 - 440. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. K. Yadav, R. R. Sudhagar, and R. Medhamurthy Apoptosis During Spontaneous and Prostaglandin F2{alpha}-Induced Luteal Regression in the Buffalo Cow (Bubalus bubalis): Involvementof Mitogen-Activated Protein Kinases Biol Reprod, September 1, 2002; 67(3): 752 - 759. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. C. Nackley, W. Shea-Eaton, D. Lopez, and M. P. McLean Repression of the Steroidogenic Acute Regulatory Gene by the Multifunctional Transcription Factor Yin Yang 1 Endocrinology, March 1, 2002; 143(3): 1085 - 1096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Zetser, T. Kisliouk, E. Ivakin, and M. Lahav Dependence on Prolactin of the Luteolytic Effect of Prostaglandin F2{alpha} in Rat Luteal Cell Cultures Biol Reprod, October 1, 2001; 65(4): 1082 - 1091. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. O. Stocco, J. Chedrese, and R. P. Deis Luteal Expression of Cytochrome P450 Side-Chain Cleavage, Steroidogenic Acute Regulatory Protein, 3{beta}-Hydroxysteroid Dehydrogenase, and 20{alpha}-Hydroxysteroid Dehydrogenase Genes in Late Pregnant Rats: Effect of Luteinizing Hormone and RU486 Biol Reprod, October 1, 2001; 65(4): 1114 - 1119. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Olson, L. E. Anderson, M. C. Wiltbank, and D. H. Townson Actions of Prostaglandin F2{{alpha}} and Prolactin on Intercellular Adhesion Molecule-1 Expression and Monocyte/Macrophage Accumulation in the Rat Corpus Luteum Biol Reprod, March 1, 2001; 64(3): 890 - 897. [Abstract] [Full Text]
|
|
|
|
|
|
N. Sekar, H. A. LaVoie, and J. D. Veldhuis Concerted Regulation of Steroidogenic Acute Regulatory Gene Expression by Luteinizing Hormone and Insulin (or Insulin-Like Growth Factor I) in Primary Cultures of Porcine Granulosa-Luteal Cells Endocrinology, November 1, 2000; 141(11): 3983 - 3992. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. P. Walsh and D. M. Stocco Effects of Lindane on Steroidogenesis and Steroidogenic Acute Regulatory Protein Expression Biol Reprod, April 1, 2000; 63(4): 1024 - 1033. [Abstract] [Full Text]
|
|
|
|
 |
|
 X. Wang, L. P. Walsh, A. J. Reinhart, and D. M. Stocco The Role of Arachidonic Acid in Steroidogenesis and Steroidogenic Acute Regulatory (StAR) Gene and Protein Expression J. Biol. Chem., June 23, 2000; 275(26): 20204 - 20209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. O. Stocco, L. Zhong, Y. Sugimoto, A. Ichikawa, L. F. Lau, and G. Gibori Prostaglandin F2alpha -induced Expression of 20alpha -Hydroxysteroid Dehydrogenase Involves the Transcription Factor NUR77 J. Biol. Chem., November 17, 2000; 275(47): 37202 - 37211. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
