| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011
Ovarian androgen production is rate limiting for follicular maturation and can induce follicular atresia. Thus, it is important to define the actions of the intraovarian agents, such as apolipoprotein (apo) E, that modulate theca cell androgen production. Theca cell androgen production is stimulated at low concentrations and inhibited at higher concentrations of native apo E. The apo E peptide, acetyl-Y(LRKLRKRLLRDADDL)2C or acetyl-Y(141–155)2C, has low density lipoprotein (LDL) receptor and LDL receptor-related protein-binding activity, and it mimics the activity of native apo E in the theca-interstitial cell system. To define the role of members of the LDL receptor superfamily in the apo E peptide-mediated responses, we found that receptor-associated protein prevented the stimulation without altering the inhibition of androstenedione production. The apo E peptide (129–162), which has no LDL receptor-binding activity, did not stimulate androstenedione production. The apo E peptide acetyl-Y(141–155)2C did not stimulate androstenedione production when cell surface heparan sulfate proteoglycans were degraded with heparinase. The apo E peptide acetyl-Y(141–155)2C bound to heparin, a property of LDL receptor ligands, and in this complex the peptide had no effect on androstenedione production. These observations support the conclusion that apo E-mediated stimulation, but not inhibition, of ovarian theca cell androstenedione production was mediated by members of the LDL receptor superfamily.
2 Correspondence: Celina V. Zerbinatti, Department of Biological Sciences, South Beaver St., Room 420, Box 5640, Northern Arizona University, Flagstaff, AZ 86011–5640. FAX: 520 523 7500; celina.zerbinatti{at}nau.edu
This article has been cited by other articles:
|
|
 |
|
  T. Fayad, R. Lefebvre, J. Nimpf, D. W. Silversides, and J. G. Lussier Low-Density Lipoprotein Receptor-Related Protein 8 (LRP8) Is Upregulated in Granulosa Cells of Bovine Dominant Follicle: Molecular Characterization and Spatio-Temporal Expression Studies Biol Reprod, March 1, 2007; 76(3): 466 - 475. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Argov, U. Moallem, and D. Sklan Lipid Transport in the Developing Bovine Follicle: Messenger RNA Expression Increases for Selective Uptake Receptors and Decreases for Endocytosis Receptors Biol Reprod, August 1, 2004; 71(2): 479 - 485. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Fayad, V. Levesque, J. Sirois, D. W. Silversides, and J. G. Lussier Gene Expression Profiling of Differentially Expressed Genes in Granulosa Cells of Bovine Dominant Follicles Using Suppression Subtractive Hybridization Biol Reprod, February 1, 2004; 70(2): 523 - 533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Sisco, L. J. Hagemann, A. N. Shelling, and P. L. Pfeffer Isolation of Genes Differentially Expressed in Dominant and Subordinate Bovine Follicles Endocrinology, September 1, 2003; 144(9): 3904 - 3913. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Raber, G. Bongers, A. LeFevour, M. Buttini, and L. Mucke Androgens Protect against Apolipoprotein E4-Induced Cognitive Deficits J. Neurosci., June 15, 2002; 22(12): 5204 - 5209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. V. Zerbinatti, L. P. Mayer, R. G. Audet, and C. A. Dyer Apolipoprotein E Is a Putative Autocrine Regulator of the Rat Ovarian Theca Cell Compartment Biol Reprod, April 1, 2001; 64(4): 1080 - 1089. [Abstract] [Full Text]
|
|
|
|
 |
|
 W. Stockinger, C. Brandes, D. Fasching, M. Hermann, M. Gotthardt, J. Herz, W. J. Schneider, and J. Nimpf The Reelin Receptor ApoER2 Recruits JNK-interacting Proteins-1 and -2 J. Biol. Chem., August 11, 2000; 275(33): 25625 - 25632. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Swertfeger and D. Y. Hui Apolipoprotein E Receptor Binding Versus Heparan Sulfate Proteoglycan Binding in Its Regulation of Smooth Muscle Cell Migration and Proliferation J. Biol. Chem., June 29, 2001; 276(27): 25043 - 25048. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
