| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a Departments of Microbiology & Immunology and
b Obstetrics & Gynecology, Wright State University School of Medicine, Dayton, Ohio 45435
Differentiation of human placental trophoblast is characterized by a process during which mononuclear villous cytotrophoblasts fuse to form a multinucleate syncytium. This event is associated with dramatic changes in gene expression. In the present study, we have applied a sensitive approach—differential display analysis—to evaluate changes in gene expression during in vitro forskolin-induced differentiation of a model of human trophoblast, the choriocarcinoma BeWo. We identified seven genes that were up-regulated; their expression and function have not previously been reported in trophoblast. Four up-regulated genes were novel upon comparison of their sequences to the GenBank database. The other three genes encode human cytochrome p450 IIC, inosine monophosphate dehydrogenase type II, and reducing agent and tunicamycin-responsive protein (RTP). Northern blot analysis revealed that RTP mRNA expression was induced to 3-fold in BeWo after 24-h incubation with forskolin and increased up to 11-fold by 72 h of forskolin treatment. The expression pattern of RTP was further investigated by in situ hybridization on second trimester and term placenta tissues. RTP mRNA was predominantly expressed in syncytiotrophoblasts in both second trimester and term placentae. The expression of RTP gene in BeWo cells was protein kinase C dependent. This is the first description of RTP gene expression in placenta and the first study elucidating the signaling pathway involved in the regulation of RTP gene expression. These results suggest that RTP may play a role in trophoblast cell proliferation and differentiation.
2 Correspondence. FAX: 937 775 2012; neal.rote{at}wright.edu
This article has been cited by other articles:
|
|
 |
|
  T. Okuda, K. Kokame, and T. Miyata Differential Expression Patterns of NDRG Family Proteins in the Central Nervous System J. Histochem. Cytochem., February 1, 2008; 56(2): 175 - 182. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. P. Kimura, D. Sizova, S. Handwerger, N. E. Cooke, and S. A. Liebhaber Epigenetic Activation of the Human Growth Hormone Gene Cluster during Placental Cytotrophoblast Differentiation Mol. Cell. Biol., September 15, 2007; 27(18): 6555 - 6568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Chen, D. M. Nelson, and Y. Sadovsky N-Myc Down-regulated Gene 1 Modulates the Response of Term Human Trophoblasts to Hypoxic Injury J. Biol. Chem., February 3, 2006; 281(5): 2764 - 2772. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Stein, E. K. Thomas, B. Herzog, M. D. Westfall, J. V. Rocheleau, R. S. Jackson II, M. Wang, and P. Liang NDRG1 Is Necessary for p53-dependent Apoptosis J. Biol. Chem., November 19, 2004; 279(47): 48930 - 48940. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Okuda, Y. Higashi, K. Kokame, C. Tanaka, H. Kondoh, and T. Miyata Ndrg1-Deficient Mice Exhibit a Progressive Demyelinating Disorder of Peripheral Nerves Mol. Cell. Biol., May 1, 2004; 24(9): 3949 - 3956. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. R. Bertani, C. D. Gladney, R. K. Johnson, and D. Pomp Evaluation of gene expression in pigs selected for enhanced reproduction using differential display PCR: II. Anterior pituitary J Anim Sci, January 1, 2004; 82(1): 32 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Handwerger and B. Aronow Dynamic Changes in Gene Expression During Human Trophoblast Differentiation Recent Prog. Horm. Res., January 1, 2003; 58(1): 263 - 281. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Boulkroun, M. Fay, M.-C. Zennaro, B. Escoubet, F. Jaisser, M. Blot-Chabaud, N. Farman, and N. Courtois-Coutry Characterization of Rat NDRG2 (N-Myc Downstream Regulated Gene 2), a Novel Early Mineralocorticoid-specific Induced Gene J. Biol. Chem., August 23, 2002; 277(35): 31506 - 31515. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. J. ARONOW, B. D. RICHARDSON, and S. HANDWERGER Microarray analysis of trophoblast differentiation: gene expression reprogramming in key gene function categories Physiol Genomics, July 17, 2001; 6(2): 105 - 116. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
