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Glyceraldehyde-3-Phosphate Dehydrogenase Expression During Apoptosis and Proliferation of Rat Ventral Prostate¹

^a Department of Medicine, Baylor College of Medicine and VA Medical Center, Houston, Texas 77030 ^b Department of Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205 ^c Johns Hopkins Oncology Center, Baltimore, Maryland 21231

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional enzyme known to play a critical role in neuronal apoptosis. We undertook the current studies to determine whether GAPDH also plays a role in prostate epithelial cell apoptosis in response to androgen deprivation. To do so, we analyzed GAPDH staining by immunohistochemistry during castration-induced involution and androgen-induced regeneration of rat ventral prostate. We found that GAPDH was undetectable in secretory epithelial cells at baseline and that staining did not increase in the epithelium during the period of peak apoptosis from 1 to 3 days after castration. However, GAPDH levels did increase within nuclei of some basal epithelial cells 5 days after castration and within the cytoplasm of all secretory epithelial cells 7 days after castration. GAPDH was also abundant within the cytoplasm of secretory epithelial cells during the period of maximal cell proliferation from 2 to 3 days after androgen replacement and was clearly apparent within nuclei of some epithelial cells 4 days after androgen replacement. Our studies suggest that GAPDH plays multiple roles during prostate epithelial cell apoptosis and proliferation.

¹ Supported by the Veteran's Administration, American Cancer Society (PRTA-14), National Institutes of Health (1 R29CA78355-01), and The Chao Fund, Baylor College of Medicine.

² Correspondence: Daniel E. Epner, VA Medical Center, Medical Service (111H), 2002 Holcombe Blvd., Houston, TX 77030. FAX: 713 794-7938; depner{at}bcm.tmc.edu

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