HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Kato, Y. Articles by Tsunoda, Y. Search for Related Content PubMed PubMed Citation Articles by Kato, Y. Articles by Tsunoda, Y. Agricola Articles by Kato, Y. Articles by Tsunoda, Y.

Developmental Potential of Mouse Follicular Epithelial Cells and Cumulus Cells After Nuclear Transfer¹

^a Laboratory of Animal Reproduction, College of Agriculture, and Research Institute for Animal Developmental Biotechnology, ^b Kinki University, Nara 631–8505, Japan ^c Molecular Oncology, Graduate School of Biological Sciences, Nara Institute of Science and Technology, Nara 630–0101, Japan

The developmental potential after nuclear transfer of mouse follicular epithelial cells cultured in vitro was examined. Follicular epithelial cells surrounding growing oocytes (type 5, diameter of oocytes, 62.6 ± 5.9 µm; n = 14) were obtained from ovaries of adult mice. Before nuclear transfer, cells were cultured for several passages and subjected to serum starvation for several days. When the nuclear-transfer oocytes were at the 2-cell stage, serial nuclear transfer was performed. Additionally, cumulus cells surrounding ovulated oocytes were used as nuclear donors, with or without thermal stimulation (from -25°C to 60°C for 10 min) before nuclear transfer. Nuclear-transfer oocytes with follicular epithelial cells developed into blastocysts (34%) after serial nuclear transfer, and 4 living fetuses on Day 10.5 (25%, 16 transferred) and 1 dead fetus on Day 19.5 of pregnancy (3%, 30 transferred) were obtained after transfer to recipients. Although blastocysts (20%) were obtained after serial nuclear transfer of cumulus cells, only one implantation site without a fetus was observed on Day 10.5 of pregnancy. Thermal stimulation of cumulus cells before nuclear transfer did not enhance the ability to develop into fetuses or blastocysts.

¹ This work was supported by grants from the Program for Promotion of Basic Research Activities for Innovative Biosciences (PROBRAIN), the Special Coordination Funds for Promoting Science and Technology from the Science and Technology Agency, and Grant-in-Aid for Encouragement of Young Scientists from The Ministry of Education, Science, Sports and Culture (09760259).

² Correspondence: Yoko Kato, Lab. of Animal Reproduction, College of Agriculture, Kinki University, 3327–204, Nakamachi, Nara 631–8505, Japan. FAX: 81 742 43 1155; yoko{at}nara.kindai.ac.jp

This article has been cited by other articles:

				S.-Z. Liu, L.-J. Yao, M.-X. Jiang, Z.-L. Lei, L.-S. Zhang, Y.-L. Zhang, Q.-Y. Sun, Y.-L. Zheng, X.-F. Song, and D.-Y. Chen Apoptosis in rabbit embryos produced by fertilization or nuclear transfer with fibroblasts and cumulus cells Reproduction, September 1, 2005; 130(3): 359 - 366. [Abstract] [Full Text] [PDF]

				S. Gao, Y. G. Chung, J. W. Williams, J. Riley, K. Moley, and K. E. Latham Somatic Cell-Like Features of Cloned Mouse Embryos Prepared with Cultured Myoblast Nuclei Biol Reprod, July 1, 2003; 69(1): 48 - 56. [Abstract] [Full Text] [PDF]

				Y.H. Choi, C.C. Love, Y.G. Chung, D.D. Varner, M.E. Westhusin, R.C. Burghardt, and K. Hinrichs Production of Nuclear Transfer Horse Embryos by Piezo-Driven Injection of Somatic Cell Nuclei and Activation with Stallion Sperm Cytosolic Extract Biol Reprod, August 1, 2002; 67(2): 561 - 567. [Abstract] [Full Text] [PDF]

				P. Loi, M. Clinton, B. Barboni, J. Fulka Jr., P. Cappai, R. Feil, R. M. Moor, and G. Ptak Nuclei of Nonviable Ovine Somatic Cells Develop into Lambs after Nuclear Transplantation Biol Reprod, July 1, 2002; 67(1): 126 - 132. [Abstract] [Full Text] [PDF]

				A. Ogura, K. Inoue, N. Ogonuki, A. Noguchi, K. Takano, R. Nagano, O. Suzuki, J. Lee, F. Ishino, and J. Matsuda Production of Male Cloned Mice from Fresh, Cultured, and Cryopreserved Immature Sertoli Cells Biol Reprod, June 1, 2000; 62(6): 1579 - 1584. [Abstract] [Full Text]