Biol Reprod
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Biology of Reproduction 61, 912-920 (1999)
©Copyright 1999 Society for the Study of Reproduction, Inc.

Articles

Efficient Human Sperm Pronucleus Formation and Replication in Xenopus Egg Extracts1

Evelyn Neuber3,a,b,c, Evis Havari3,a, J. Aquiles Sancheza, R. Douglas Powersb,c, and Lawrence J. Wangh2,a

a Department of Biology, Brandeis University, Waltham, Massachusetts 02454 b Department of Biology, Boston College, Chestnut Hill, Massachusetts 02167 c Boston Fertility Laboratories, Brookline, Massachusetts 02146

We have achieved efficient in vitro reactivation and replication of human sperm nuclei in frog egg extracts by constructing a 4-step protocol that mimics the events of fertilization and pronucleus formation in mammalian eggs. With use of this protocol, 78–97% of human sperm nuclei from fertile donors synchronously swelled and completed full genome replication in about 2 h. We document the changes in nuclear structure that accompany efficient DNA synthesis and discuss future research and potential clinical implications of this new system.

1 Supported by Boston IVF Inc. and Hamilton Thorne Research Inc.

2 Correspondence: L.J. Wangh, Department of Biology, Brandeis University, 415 South Street, Waltham, MA 02454-9110. FAX: 781 736 3107; wangh{at}brandeis.edu

3 These authors contributed equally to this work.






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Copyright © 1999 by the Society for the Study of Reproduction.