| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a Department of Biology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China
Activin plays important roles in the regulation of vertebrate reproduction. Using zebrafish, Danio rerio, as a model, the present study aimed at investigating the role of activin in the regulation of final oocyte maturation. Administration of recombinant goldfish activin B significantly increased the rate of oocyte maturation in vitro in a dose- and time-dependent manner. The effect of activin seemed to be additive to the effects of gonadotropin (hCG) and 17
,20ß-dihydroxyprogesterone, a potent maturation-inducing hormone in teleosts. The specificity of the activin action was confirmed by coincubation with recombinant human follistatin, which completely abolished the stimulatory effect of activin B. Interestingly, follistatin also significantly inhibited hCG-induced oocyte maturation, suggesting that endogenous activin may be a downstream mediator of gonadotropin actions. No effect of activin B was observed in the presence of actinomycin D, indicating that the action of activin may involve changes in transcriptional activity. These results, together with the demonstration that activin and its type II receptor are expressed in the zebrafish ovary, strongly suggest a paracrine/autocrine role for activin in the controlling of final oocyte maturation.
2 Correspondence: Wei Ge, Department of Biology, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China. FAX: 852 2609 5646; weige{at}cuhk.edu.hk
This article has been cited by other articles:
|
|
 |
|
  S. Seki, T. Kouya, R. Tsuchiya, D. M Valdez Jr, B. Jin, T. Hara, N. Saida, M. Kasai, and K. Edashige Development of a reliable in vitro maturation system for zebrafish oocytes Reproduction, March 1, 2008; 135(3): 285 - 292. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. S. Clelland, Q. Tan, A. Balofsky, R. Lacivita, and C. Peng Inhibition of Premature Oocyte Maturation: A Role for Bone Morphogenetic Protein 15 in Zebrafish Ovarian Follicles Endocrinology, November 1, 2007; 148(11): 5451 - 5458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Clelland, G. Kohli, R. K. Campbell, S. Sharma, S. Shimasaki, and C. Peng Bone Morphogenetic Protein-15 in the Zebrafish Ovary: Complementary Deoxyribonucleic Acid Cloning, Genomic Organization, Tissue Distribution, and Role in Oocyte Maturation Endocrinology, January 1, 2006; 147(1): 201 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W.-K. So, H.-F. Kwok, and W. Ge Zebrafish Gonadotropins and Their Receptors: II. Cloning and Characterization of Zebrafish Follicle-Stimulating Hormone and Luteinizing Hormone Subunits--Their Spatial-Temporal Expression Patterns and Receptor Specificity Biol Reprod, June 1, 2005; 72(6): 1382 - 1396. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-F. Kwok, W.-K. So, Y. Wang, and W. Ge Zebrafish Gonadotropins and Their Receptors: I. Cloning and Characterization of Zebrafish Follicle-Stimulating Hormone and Luteinizing Hormone Receptors-- Evidence for Their Distinct Functions in Follicle Development Biol Reprod, June 1, 2005; 72(6): 1370 - 1381. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang and W. Ge Developmental Profiles of Activin {beta}A, {beta}B, and Follistatin Expression in the Zebrafish Ovary: Evidence for Their Differential Roles During Sexual Maturation and Ovulatory Cycle Biol Reprod, December 1, 2004; 71(6): 2056 - 2064. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.-T. Wong and Y. Zohar Novel Expression of Gonadotropin Subunit Genes in Oocytes of the Gilthead Seabream (Sparus aurata) Endocrinology, November 1, 2004; 145(11): 5210 - 5220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang and W. Ge Cloning of Epidermal Growth Factor (EGF) and EGF Receptor from the Zebrafish Ovary: Evidence for EGF as a Potential Paracrine Factor from the Oocyte to Regulate Activin/Follistatin System in the Follicle Cells Biol Reprod, September 1, 2004; 71(3): 749 - 760. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang and W. Ge Spatial Expression Patterns of Activin and Its Signaling System in the Zebrafish Ovarian Follicle: Evidence for Paracrine Action of Activin on the Oocytes Biol Reprod, December 1, 2003; 69(6): 1998 - 2006. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang, A. O. L. Wong, and W. Ge Cloning, Regulation of Messenger Ribonucleic Acid Expression, and Function of a New Isoform of Pituitary Adenylate Cyclase-Activating Polypeptide in the Zebrafish Ovary Endocrinology, November 1, 2003; 144(11): 4799 - 4810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Wang and W. Ge Involvement of Cyclic Adenosine 3',5'-Monophosphate in the Differential Regulation of Activin {beta}A and {beta}B Expression by Gonadotropin in the Zebrafish Ovarian Follicle Cells Endocrinology, February 1, 2003; 144(2): 491 - 499. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. M. Spitsbergen and M. L. Kent The State of the Art of the Zebrafish Model for Toxicology and Toxicologic Pathology Research--Advantages and Current Limitations Toxicol Pathol, January 1, 2003; 31(1_suppl): 62 - 87. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Welt, Y. Sidis, H. Keutmann, and A. Schneyer Activins, Inhibins, and Follistatins: From Endocrinology to Signaling. A Paradigm for the New Millennium Experimental Biology and Medicine, October 1, 2002; 227(9): 724 - 752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. E. Drummond, M. T. Le, J.-F. Ethier, M. Dyson, and J. K. Findlay Expression and Localization of Activin Receptors, Smads, and {beta}glycan to the Postnatal Rat Ovary Endocrinology, April 1, 2002; 143(4): 1423 - 1433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Pang and W. Ge Gonadotropin and Activin Enhance Maturational Competence of Oocytes in the Zebrafish (Danio rerio) Biol Reprod, February 1, 2002; 66(2): 259 - 265. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Pang and W. Ge Epidermal Growth Factor and TGF{alpha} Promote Zebrafish Oocyte Maturation in Vitro: Potential Role of the Ovarian Activin Regulatory System Endocrinology, January 1, 2002; 143(1): 47 - 54. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Wu, H. Patel, S. Mukai, C. Melino, R. Garg, X. Ni, J. Chang, and C. Peng Activin, Inhibin, and Follistatin in Zebrafish Ovary: Expression and Role in Oocyte Maturation Biol Reprod, June 1, 2000; 62(6): 1585 - 1592. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
