| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
Stimulates Proliferation of Rat Ovarian Theca-Interstitial Cells1
a Karol Marcinkowski University of Medical Sciences, Department of Gynecology and Obstetrics, 60-535 Poznan, Poland
b Yale University School of Medicine, Department of Obstetrics and Gynecology, New Haven, Connecticut 06520-8063
Tumor necrosis factor-
(TNF-) is a potent modulator of ovarian function, affecting steroidogenesis of both granulosa and theca-interstitial (T-I) cells. Women with polycystic ovary syndrome (PCOS) have increased levels of serum TNF-. The present study evaluated the effects of TNF- on T-I cell proliferation. Purified rat T-I cells were cultured for 48 h with or without TNF- (0.001–1 nM), insulin-like growth factor I (IGF-I; 10 nM), and/or insulin (10 nM). Proliferation was measured by [3H]thymidine incorporation assay and by counting the steroidogenically active (stained positive for 3ß-hydroxysteroid dehydrogenase; 3ß-HSD) and inactive (3ß-HSD negative) cells. TNF- stimulated thymidine incorporation in a dose-dependent fashion (up to 3.2-fold; P < 0.01). Insulin and IGF-I stimulated T-I proliferation (respectively, by up to 2.4- and 3.1-fold; P < 0.001). TNF- potentiated effects of insulin and IGF-I in a dose-dependent and additive fashion (up to 6.7-fold; P < 0.001). TNF- (1 nM) increased total cell count (by 80%, P < 0.05) and the proportion of 3ß-HSD-positive cells (by 19%, P < 0.05). Flow cytometry DNA analysis revealed that TNF- (1 nM) increased the proliferative index by up to 16% (P = 0.05). The present findings demonstrate that TNF- stimulates mitotic activity of T-I cells by increasing the proportion of actively dividing cells and preferentially increasing the number of steroidogenically active cells. The effects of TNF- appear to be independent of those induced by insulin and IGF-I. We postulate that TNF- may play a pathophysiologic role in disorders of the T-I compartment, such as PCOS.
2 Correspondence: Antoni J. Duleba, Yale University School of Medicine, Department of Obstetrics and Gynecology, 333 Cedar Street, New Haven, CT 06520. FAX: 203 785 7134; antoni.duleba{at}yale.edu
This article has been cited by other articles:
|
|
 |
|
  E. H. Ruder, T. J. Hartman, J. Blumberg, and M. B. Goldman Oxidative stress and antioxidants: exposure and impact on female fertility Hum. Reprod. Update, June 4, 2008; (2008) dmn011v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Skarzynski, I. Woclawek-Potocka, A. Korzekwa, M. M. Bah, K. Piotrowska, B. Barszczewska, and K. Okuda Infusion of Exogenous Tumor Necrosis Factor Dose Dependently Alters the Length of the Luteal Phase in Cattle: Differential Responses to Treatment with Indomethacin and L-NAME, a Nitric Oxide Synthase Inhibitor Biol Reprod, April 1, 2007; 76(4): 619 - 627. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. V. Mikhaylova, T. Kuulasmaa, J. Jaaskelainen, and R. Voutilainen Tumor Necrosis Factor-{alpha} Regulates Steroidogenesis, Apoptosis, and Cell Viability in the Human Adrenocortical Cell Line NCI-H295R Endocrinology, January 1, 2007; 148(1): 386 - 392. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Gonzalez, N. S. Rote, J. Minium, and J. P. Kirwan Increased Activation of Nuclear Factor {kappa}B Triggers Inflammation and Insulin Resistance in Polycystic Ovary Syndrome J. Clin. Endocrinol. Metab., April 1, 2006; 91(4): 1508 - 1512. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F Gonzalez, N S Rote, J Minium, and J P Kirwan In vitro evidence that hyperglycemia stimulates tumor necrosis factor-{alpha} release in obese women with polycystic ovary syndrome. J. Endocrinol., March 1, 2006; 188(3): 521 - 529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Sander, C. G. Luchetti, M. E. Solano, E. Elia, G. Di Girolamo, C. Gonzalez, and A. B. Motta Role of the N, N'-dimethylbiguanide metformin in the treatment of female prepuberal BALB/c mice hyperandrogenized with dehydroepiandrosterone. Reproduction, March 1, 2006; 131(3): 591 - 602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Gonzalez, N. S. Rote, J. Minium, and J. P. Kirwan Reactive Oxygen Species-Induced Oxidative Stress in the Development of Insulin Resistance and Hyperandrogenism in Polycystic Ovary Syndrome J. Clin. Endocrinol. Metab., January 1, 2006; 91(1): 336 - 340. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. F. Escobar-Morreale, M. Luque-Ramirez, and J. L. San Millan The Molecular-Genetic Basis of Functional Hyperandrogenism and the Polycystic Ovary Syndrome Endocr. Rev., April 1, 2005; 26(2): 251 - 282. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.J. Duleba, N. Foyouzi, M. Karaca, T. Pehlivan, J. Kwintkiewicz, and H.R. Behrman Proliferation of ovarian theca-interstitial cells is modulated by antioxidants and oxidative stress Hum. Reprod., July 1, 2004; 19(7): 1519 - 1524. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. J. Skarzynski, M. M. Bah, K. M. Deptula, I. Woclawek-Potocka, A. Korzekwa, M. Shibaya, W. Pilawski, and K. Okuda Roles of Tumor Necrosis Factor-{alpha} of the Estrous Cycle in Cattle: An In Vivo Study Biol Reprod, December 1, 2003; 69(6): 1907 - 1913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Ledee-Bataille, G. Lapree-Delage, J.L. Taupin, S. Dubanchet, J. Taieb, J.F. Moreau, and G. Chaouat Follicular fluid concentration of leukaemia inhibitory factor is decreased among women with polycystic ovarian syndrome during assisted reproduction cycles Hum. Reprod., October 1, 2001; 16(10): 2073 - 2078. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. F. Escobar-Morreale, R. M. Calvo, J. Sancho, and J. L. San Millan TNF-{alpha} and Hyperandrogenism: A Clinical, Biochemical, and Molecular Genetic Study J. Clin. Endocrinol. Metab., August 1, 2001; 86(8): 3761 - 3767. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
