| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a Endocrinology and Reproductive Physiology Program, Department of Animal Sciences, University of Wisconsin-Madison, Madison, Wisconsin 53706
The objective of this study was to examine the timing and mechanisms involved in transcription initiation in bovine embryos. Transcriptional activity and its regulation were explored by labeling 1-cell zygotes and 2-cell embryos with [3H]uridine in the presence or absence of alpha-amanitin, aphidicolin, and tricostatin A (TSA) (inhibitors of mRNA synthesis, DNA replication, and histone deacetylases, respectively) followed by a total RNA isolation and determination of [3H]uridine incorporation. We also analyzed translation of zygotic/embryonic mRNAs by labeling zygotes and 2-cell embryos with [35S]methionine in the presence or absence of alpha-amanitin, aphidicolin, and TSA followed by two-dimensional PAGE and autoradiography. We show that bovine 1-cell zygotes and 2-cell embryos are transcriptionally and translationally active. The first and second rounds of DNA replication are important regulators of early gene expression as the inhibition of DNA replication resulted in a dramatic decrease in both transcriptional and translational activity. Moreover, acetylation of histones plays an important role in this early gene activation at the onset of embryonic development in the cow.
2 Correspondence: Neal L. First, University of Wisconsin-Madison, Department of Animal Sciences, 1675 Observatory Drive, Madison, WI 53706. FAX: 608 262 5157; nlf{at}calshp.cals.wisc.edu
3 Current address: Harvard Medical School, Harvard Institute of Human Genetics, 4 Blackfan Circle, Boston, MA 02115.
This article has been cited by other articles:
|
|
 |
|
  A Bonnet, R Dalbies-Tran, and M A Sirard Opportunities and challenges in applying genomics to the study of oogenesis and folliculogenesis in farm animals Reproduction, February 1, 2008; 135(2): 119 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E Memili, D Peddinti, L A Shack, B Nanduri, F McCarthy, H Sagirkaya, and S C Burgess Bovine germinal vesicle oocyte and cumulus cell proteomics Reproduction, June 1, 2007; 133(6): 1107 - 1120. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. V Patel, A. Bettegowda, J. J Ireland, P. M Coussens, P. Lonergan, and G. W Smith Functional genomics studies of oocyte competence: evidence that reduced transcript abundance for follistatin is associated with poor developmental competence of bovine oocytes Reproduction, January 1, 2007; 133(1): 95 - 106. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Misirlioglu, G. P. Page, H. Sagirkaya, A. Kaya, J. J. Parrish, N. L. First, and E. Memili Dynamics of global transcriptome in bovine matured oocytes and preimplantation embryos PNAS, December 12, 2006; 103(50): 18905 - 18910. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-y. Yang, H. Li, Q.-w. Ma, J.-b. Yan, J.-g. Zhao, H.-w. Li, H.-q. Shen, H.-f. Liu, Y. Huang, S.-Z. Huang, et al. Improved efficiency of bovine cloning by autologous somatic cell nuclear transfer. Reproduction, November 1, 2006; 132(5): 733 - 739. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Sagirkaya, M. Misirlioglu, A. Kaya, N. L First, J. J Parrish, and E. Memili Developmental and molecular correlates of bovine preimplantation embryos. Reproduction, May 1, 2006; 131(5): 895 - 904. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Lloyd, J.-H. Lee, R. Alberio, E. J. Bowles, J. Ramalho-Santos, K. H. S. Campbell, and J. C. St. John Aberrant Nucleo-cytoplasmic Cross-Talk Results in Donor Cell mtDNA Persistence in Cloned Embryos Genetics, April 1, 2006; 172(4): 2515 - 2527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Vallee, C. Gravel, M.-F. Palin, H. Reghenas, P. Stothard, D. S. Wishart, and M.-A. Sirard Identification of Novel and Known Oocyte-Specific Genes Using Complementary DNA Subtraction and Microarray Analysis in Three Different Species Biol Reprod, July 1, 2005; 73(1): 63 - 71. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Oropeza, C. Wrenzycki, D. Herrmann, K.-G. Hadeler, and H. Niemann Improvement of the Developmental Capacity of Oocytes from Prepubertal Cattleby Intraovarian Insulin-Like Growth Factor-I Application Biol Reprod, June 1, 2004; 70(6): 1634 - 1643. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Vigneault, S. McGraw, L. Massicotte, and M.-A. Sirard Transcription Factor Expression Patterns in Bovine In Vitro-Derived Embryos Priorto Maternal-Zygotic Transition Biol Reprod, June 1, 2004; 70(6): 1701 - 1709. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Gao, E. Czirr, Y. G. Chung, Z. Han, and K. E. Latham Genetic Variation in Oocyte Phenotype Revealed Through Parthenogenesis and Cloning: Correlation with Differences in Pronuclear Epigenetic Modification Biol Reprod, April 1, 2004; 70(4): 1162 - 1170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.S. Lequarre, J. Marchandise, B. Moreau, A. Massip, and I. Donnay Cell Cycle Duration at the Time of Maternal Zygotic Transition for In Vitro Produced Bovine Embryos: Effect of Oxygen Tension and Transcription Inhibition Biol Reprod, November 1, 2003; 69(5): 1707 - 1713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Comizzoli, F. Urner, D. Sakkas, and J.P. Renard Up-Regulation of Glucose Metabolism During Male Pronucleus Formation Determines the Early Onset of the S Phase in Bovine Zygotes Biol Reprod, May 1, 2003; 68(5): 1934 - 1940. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Ramalho-Santos, P. Sutovsky, C. Simerly, R. Oko, G. M. Wessel, L. Hewitson, and G. Schatten ICSI choreography: fate of sperm structures after monospermic rhesus ICSI and first cell cycle implications Hum. Reprod., December 1, 2000; 15(12): 2610 - 2620. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
