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a Research Laboratories of Schering AG, Berlin, Germany
b Health Care Discovery of Novo Nordisk A/S, Copenhagen, Denmark
c Department of Anatomy and Reproductive Biology, School of Medicine, RWTH University of Aachen, D-52057 Aachen, Germany
d Fakultät für Biologie, Gentechnologie/Biotechnologie, University Bielefeld, D-33501 Bielefeld, Germany
We also investigated the influence of FF-MAS on chromosome, microtubule, and ultrastructural dynamics in Hx-cultured oocytes by immunocytochemistry and electron microscopy. Similarly to spontaneously matured oocytes, chromosomes became aligned, a barrel-shaped spindle formed, and overall organelle distribution was normal in FF-MAS-matured oocytes. The number of small cytoplasmic asters was elevated in FF-MAS-treated oocytes. Although the number of cortical granules (CGs) was similar to that in spontaneously matured oocytes, the overall distance between CGs and oolemma was increased in the FF-MAS group. These observations suggest that the initiation of meiotic maturation in FF-MAS-treated oocytes in the presence of high cAMP levels leads to a delayed but otherwise normal nuclear maturation. FF-MAS appears to improve oocyte quality by supporting microtubule assembly and by delaying CG release, which is known to contribute to reduced fertilization.
Synthetically produced meiosis-activating sterol, a sterol originally derived from follicular fluid (FF-MAS), induces meiotic maturation of mouse oocytes in vitro. We therefore compared FF-MAS-induced maturation of naked mouse oocytes arrested in prophase I by either hypoxanthine (Hx) or forskolin (Fo) with spontaneous maturation of naked oocytes. FF-MAS-treated oocytes overcame the meiotic block by Hx or Fo, although germinal vesicle breakdown was delayed by 11 h and 7 h, respectively.
2 Correspondence: Christa Hegele-Hartung, FC/HT Research of Schering AG, Müllerstrasse 170–178, D-13342 Berlin, Germany. FAX: 49 30 46818056; christa.hegelehartung{at}schering.de
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