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Fertility of Y^TIR.B6 Sex-Reversal Females with XX Orthotopic Ovarian Transplants¹

^a Instituto de Investigaciones Biomédicas, Universidad Nacional Autonoma de Mexico, 04510 México, D.F., Mexico ^b Department of Biology, McGill University, Montreal, Quebec, Canada H3A 1B1

When the Y chromosome of Mus musculus domesticus (Y^TIR) was introduced onto the C57BL/6J (B6) mouse background, testis development was impaired and half of the XY progeny (Y^TIR.B6) developed a female phenotype. Y^TIR.B6 fetal ovaries showed massive death of medullary oocytes and, after birth, produced abnormal levels of steroid hormones, exhibited irregular estrous cycles, and failed to become fertile. In this study we examined whether alterations during perinatal development observed in Y^TIR.B6 ovaries permanently impaired the establishment of the hypothalamus-pituitary-ovary axis (HPOa). B6 fetal and postnatal ovaries at different stages (fetal, infantile, or adult) were transplanted orthotopically (to the ovarian bursa) to either ovariectomized B6 normal females or Y^TIR.B6 sex-reversal females. Percentage of pregnancy, litter size, and capacity to feed pups were recorded. Reciprocally, XY^TIR.B6 ovaries were orthotopically transplanted into B6 females. After crossing with fertile males, several Y^TIR.B6 sex-reversal females with B6 ovarian transplants at all ages became pregnant, had offspring, and fed their pups. On the other hand, none of the B6 female hosts with XY^TIR ovaries became pregnant. Results demonstrated that Y^TIR.B6 sex-reversal females maintain a functional HPOa and that their failure to reproduce is primarily due to an ovarian defect.

¹ Supported by grant IN204598 from PAPIIT DGAPA-UNAM.

² Correspondence: H. Merchant-Larios, Department of Cell Biology, Instituto de Investigaciones Biomedicas, UNAM, Apartado Postal 70228, Ciudad Universitaria, Mexico, D.F. Mexico 04510. FAX: 525 622 3897; merchant{at}servidor.unam.mx

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