| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a Institute of Human Genetics, University of Göttingen, D-37073 Göttingen, Germany
b Institute of Genetics, University of Düsseldorf, D-40225 Düsseldorf, Germany
Cyritestin is a membrane-anchored sperm protein belonging to the ADAM (
isintegrin and 
etalloprotease) family of proteins, which are proposed to be involved in cell-cell adhesion through binding to integrin receptors. Several lines of evidence support a role of cyritestin and other members of this protein family in the fusion of sperm and the egg plasma membrane. In an effort to elucidate the physiological function of cyritestin, we have disrupted its locus by homologous recombination. Male homozygous null mutants are infertile, even though spermatogenesis, mating, and migration of sperm from the uterus into the oviduct are normal. In vitro experiments showed that infertility is due to the inability of the cyritestin-deficient sperm to bind to the zona pellucida. However, after removal of the zona pellucida, sperm-egg membrane fusion monitored by the presence of pronuclei and generation of 2- and 4-cell embryos did not reveal any differences from the wild-type situation. These results demonstrate that cyritestin is crucial in the fertilization process at the level of the sperm-zona pellucida interaction.
2 Correspondence: Wolfgang Engel, Institute of Human Genetics Gosslerstrasse 12d, D-37073 Göttingen, Germany. FAX: 49 551 399303; wengel{at}gwdg.de
This article has been cited by other articles:
|
|
 |
|
  R. Yamaguchi, Y. Muro, A. Isotani, K. Tokuhiro, K. Takumi, I. Adham, M. Ikawa, and M. Okabe Disruption of ADAM3 Impairs the Migration of Sperm into Oviduct in Mouse Biol Reprod, July 1, 2009; 81(1): 142 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Han, E. Choi, I. Park, B. Lee, S. Jin, D. H. Kim, H. Nishimura, and C. Cho Comprehensive Analysis of Reproductive ADAMs: Relationship of ADAM4 and ADAM6 with an ADAM Complex Required for Fertilization in Mice Biol Reprod, May 1, 2009; 80(5): 1001 - 1008. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Gyamera-Acheampong and M. Mbikay Proprotein convertase subtilisin/kexin type 4 in mammalian fertility: a review Hum. Reprod. Update, March 1, 2009; 15(2): 237 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P Sipila, J Jalkanen, I T Huhtaniemi, and M Poutanen Novel epididymal proteins as targets for the development of post-testicular male contraception Reproduction, March 1, 2009; 137(3): 379 - 389. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Pasten-Hidalgo, R. Hernandez-Rivas, A. L. Roa-Espitia, M. Sanchez-Gutierrez, F. Martinez-Perez, A. O. Monrroy, E. O Hernandez-Gonzalez, and A. Mujica Presence, processing, and localization of mouse ADAM15 during sperm maturation and the role of its disintegrin domain during sperm-egg binding Reproduction, July 1, 2008; 136(1): 41 - 51. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. D. Mruk, B. Silvestrini, and C. Y. Cheng Anchoring Junctions As Drug Targets: Role in Contraceptive Development Pharmacol. Rev., June 1, 2008; 60(2): 146 - 180. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Ueda, R. Yamaguchi, M. Ikawa, M. Okabe, E. Morii, Y. Maeda, and T. Kinoshita PGAP1 Knock-out Mice Show Otocephaly and Male Infertility J. Biol. Chem., October 19, 2007; 282(42): 30373 - 30380. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nishimura, D. G. Myles, and P. Primakoff Identification of an ADAM2-ADAM3 Complex on the Surface of Mouse Testicular Germ Cells and Cauda Epididymal Sperm J. Biol. Chem., June 15, 2007; 282(24): 17900 - 17907. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Yamaguchi, K. Yamagata, M. Ikawa, S. B. Moss, and M. Okabe Aberrant Distribution of ADAM3 in Sperm from Both Angiotensin-Converting Enzyme (Ace)- and Calmegin (Clgn)-Deficient Mice Biol Reprod, November 1, 2006; 75(5): 760 - 766. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Chen, X. Huang, and D. Sheppard ADAM33 Is Not Essential for Growth and Development and Does Not Modulate Allergic Asthma in Mice. Mol. Cell. Biol., September 1, 2006; 26(18): 6950 - 6956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Sipila, D. A. Pujianto, R. Shariatmadari, J. Nikkila, M. Lehtoranta, I. T. Huhtaniemi, and M. Poutanen Differential Endocrine Regulation of Genes Enriched in Initial Segment and Distal Caput of the Mouse Epididymis as Revealed by Genome-Wide Expression Profiling Biol Reprod, August 1, 2006; 75(2): 240 - 251. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Borghei, Y.-B. Ouyang, A. D. Westmuckett, M. R. Marcello, C. P. Landel, J. P. Evans, and K. L. Moore Targeted Disruption of Tyrosylprotein Sulfotransferase-2, an Enzyme That Catalyzes Post-translational Protein Tyrosine O-Sulfation, Causes Male Infertility J. Biol. Chem., April 7, 2006; 281(14): 9423 - 9431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kim, J. Oh, J.-M. Woo, E. Choi, S. H. Im, Y. J. Yoo, D. H. Kim, H. Nishimura, and C. Cho Expression and Relationship of Male Reproductive ADAMs in Mouse Biol Reprod, April 1, 2006; 74(4): 744 - 750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Kim, M. Yamashita, T. Nakanishi, K.-E. Park, M. Kimura, S.-i. Kashiwabara, and T. Baba Mouse Sperm Lacking ADAM1b/ADAM2 Fertilin Can Fuse with the Egg Plasma Membrane and Effect Fertilization J. Biol. Chem., March 3, 2006; 281(9): 5634 - 5639. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Kim, D. Baba, M. Kimura, M. Yamashita, S.-i. Kashiwabara, and T. Baba Identification of a hyaluronidase, Hyal5, involved in penetration of mouse sperm through cumulus mass PNAS, December 13, 2005; 102(50): 18028 - 18033. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Stein, J. C. Go, P. Primakoff, and D. G. Myles Defects in Secretory Pathway Trafficking During Sperm Development in Adam2 Knockout Mice Biol Reprod, November 1, 2005; 73(5): 1032 - 1038. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. K. Stein, P. Primakoff, and D. Myles Sperm-egg fusion: events at the plasma membrane J. Cell Sci., December 15, 2004; 117(26): 6269 - 6274. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Nakanishi, A. Isotani, R. Yamaguchi, M. Ikawa, T. Baba, S. S. Suarez, and M. Okabe Selective Passage Through the Uterotubal Junction of Sperm from a Mixed Population Produced by Chimeras of Calmegin-Knockout and Wild-Type Male Mice Biol Reprod, September 1, 2004; 71(3): 959 - 965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Nishimura, E. Kim, T. Nakanishi, and T. Baba Possible Function of the ADAM1a/ADAM2 Fertilin Complex in the Appearance of ADAM3 on the Sperm Surface J. Biol. Chem., August 13, 2004; 279(33): 34957 - 34962. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 U. Schlecht, P. Demougin, R. Koch, L. Hermida, C. Wiederkehr, P. Descombes, C. Pineau, B. Jegou, and M. Primig Expression Profiling of Mammalian Male Meiosis and Gametogenesis Identifies Novel Candidate Genes for Roles in the Regulation of Fertility Mol. Biol. Cell, March 1, 2004; 15(3): 1031 - 1043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Ellerman, C. Ha, P. Primakoff, D. G. Myles, and G. S. Dveksler Direct Binding of the Ligand PSG17 to CD9 Requires a CD9 Site Essential for Sperm-Egg Fusion Mol. Biol. Cell, December 1, 2003; 14(12): 5098 - 5103. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Schultz, F. K. Hamra, and D. L. Garbers A multitude of genes expressed solely in meiotic or postmeiotic spermatogenic cells offers a myriad of contraceptive targets PNAS, October 14, 2003; 100(21): 12201 - 12206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Horiuchi, G. Weskamp, L. Lum, H.-P. Hammes, H. Cai, T. A. Brodie, T. Ludwig, R. Chiusaroli, R. Baron, K. T. Preissner, et al. Potential Role for ADAM15 in Pathological Neovascularization in Mice Mol. Cell. Biol., August 15, 2003; 23(16): 5614 - 5624. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Alfieri, A. D. Martin, J. Takeda, G. Kondoh, D. G. Myles, and P. Primakoff Infertility in female mice with an oocyte-specific knockout of GPI-anchored proteins J. Cell Sci., June 1, 2003; 116(11): 2149 - 2155. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Inoue, M. Ikawa, T. Nakanishi, M. Matsumoto, M. Nomura, T. Seya, and M. Okabe Disruption of Mouse CD46 Causes an Accelerated Spontaneous Acrosome Reaction in Sperm Mol. Cell. Biol., April 1, 2003; 23(7): 2614 - 2622. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. O. Weinberg, M. Mirotsou, J. Gannon, V. J. Dzau, R. T. Lee, and R. E. Pratt Sex dependence and temporal dependence of the left ventricular genomic response to pressure overload Physiol Genomics, January 15, 2003; 12(2): 113 - 127. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Talbot, B. D. Shur, and D. G. Myles Cell Adhesion and Fertilization: Steps in Oocyte Transport, Sperm-Zona Pellucida Interactions, and Sperm-Egg Fusion Biol Reprod, January 1, 2003; 68(1): 1 - 9. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Frayne, E. A.C. Hurd, and L. Hall Human tMDC III: a sperm protein with a potential role in oocyte recognition Mol. Hum. Reprod., September 1, 2002; 8(9): 817 - 822. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Primakoff and D. G. Myles Penetration, Adhesion, and Fusion in Mammalian Sperm-Egg Interaction Science, June 21, 2002; 296(5576): 2183 - 2185. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Weskamp, H. Cai, T. A. Brodie, S. Higashyama, K. Manova, T. Ludwig, and C. P. Blobel Mice Lacking the Metalloprotease-Disintegrin MDC9 (ADAM9) Have No Evident Major Abnormalities during Development or Adult Life Mol. Cell. Biol., March 1, 2002; 22(5): 1537 - 1544. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. McLaughlin, J. Frayne, G. Bloomerg, and L. Hall Do fertilin {beta} and cyritestin play a major role in mammalian sperm-oolemma interactions? A critical re-evaluation of the use of peptide mimics in identifying specific oocyte recognition proteins Mol. Hum. Reprod., April 1, 2001; 7(4): 313 - 317. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takahashi, D. Bigler, Y. Ito, and J. M. White Sequence-Specific Interaction between the Disintegrin Domain of Mouse ADAM 3 and Murine Eggs: Role of {beta}1 Integrin-associated Proteins CD9, CD81, and CD98 Mol. Biol. Cell, April 1, 2001; 12(4): 809 - 820. [Abstract] [Full Text]
|
|
|
|
|
|
G. Zhu, D. Myles, and P Primakoff Testase 1 (ADAM 24) a plasma membrane-anchored sperm protease implicated in sperm function during epididymal maturation or fertilization J. Cell Sci., January 5, 2001; 114(9): 1787 - 1794. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
