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Evidence for the Presence of Tumor Necrosis Factor Alpha Receptors During Ovarian Development in the Rat¹

^a Department of Biological Sciences, Kent State University, Kent, Ohio 44242

Tumor necrosis factor alpha (TNF) is an intraovarian cytokine that may play a role in ovarian development and function. Effects of TNF are mediated by binding to at least one of two TNF receptor subtypes (with molecular masses of approximately 60 and 80 kDa); therefore, the overall goal of this study was to determine whether rat ovaries have TNF receptors during critical times in development. Two approaches were used: 1) demonstration of specific binding of radiolabeled TNF to ovarian cells and 2) semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) analysis for each of the two TNF receptors. Ovarian cells were obtained on Embryonic Day 19, day of birth (Day 0), and Days 2, 5, 10, and 20. TNF binding was present on all days, with significantly greater binding on Day 20. Messenger RNA for both receptor subtypes was detected on all days using RT-PCR analysis but was significantly greater for the 60-kDa receptor on Day 20. In conclusion, rat ovaries contained receptors capable of binding TNF and mRNA for both receptor subtypes. Identification of ovarian TNF receptors provides support for a role of TNF in ovarian development and function.

¹ This work was supported by NIH grant #1-R15-HD34252-01 (to J.L.M.) and preliminary reports of this work were presented to the Society for the Study of Reproduction (Biol Reprod 56: Supplement 1, Abstract #447, 1997).

² Correspondence: Jennifer L. Marcinkiewicz, 256 Cunningham Hall, Department of Biological Sciences, Kent State University, Kent, OH 44242. FAX: 330 672 3713; jmarcink{at}kent.edu

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