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The Krüppel-Like Core Promoter Binding Protein Gene Is Primarily Expressed in Placenta During Mouse Development¹

^a Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina ^b Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS/INSERM U184/ULP, BP 163 67404 Illkirch Cedex, C.U. de Strasbourg, France ^c Unite INSERM U.384, Faculté de Médecine, U.F.R. de Medecine et de Pharmacie, B.P 38 F-63001 Clermont-Ferrand Cedex, France

The human core promoter binding protein (hCPBP) has been identified as a DNA-binding protein involved in the regulation of TATA box-less genes like those encoding the pregnancy-specific glycoproteins. Structurally, hCPBP contains three zinc fingers in the C-terminal domain, which is highly conserved in a number of proteins that constitute the Krüppel-like family of transcription factors. In the present work, we report the molecular cloning of the mouse CPBP (mCPBP) and its expression pattern during development as well as in adult tissues. The mouse cDNA encodes a protein of 283 amino acids that share 94.4% of identity with the hCPBP. The highest level of mCPBP transcript was detected in placenta, and its expression was lower in total embryos and in adult tissues. We also show by in situ hybridization that during embryonic development the mCPBP gene is mainly expressed in extra-embryonic structures throughout gestation; essentially no specific expression was detected in embryonic tissues. Our data demonstrate that CPBP transcript is enriched in the trophoblastic tissue and strongly suggest that its encoded polypeptide regulates target genes involved in placental development and pregnancy maintenance.

¹ This work was supported by grants from the Third World Academy of Sciences, the Secretaría de Ciencia y Técnica-Universidad Nacional de Córdoba (SECyT), the Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), and FONCYT. V.S. is supported by an INSERM grant. J.L.B. is a career investigator of CONICET. D.S. and F.L-D are recipients of fellowships from SECyT and CONICET of Argentina.

² Correspondence: J.L. Bocco, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Pabellón Argentina—Ciudad Universitaria (5000), Cordoba, Argentina. FAX: 54 351 4334174; jbocco{at}fcq.unc.edu.ar

³ Current address: Hormone and Metabolic Research Unit, International Institute of Cellular and Molecular Pathology and University of Louvain Medical School, 75, Avenue Hippocrate, B-1200 Brussels, Belgium.

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