| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a Departments of Animal Sciences and
b Biochemistry, University of Missouri, Columbia, Missouri 65211
c Molecular Embryology Group, AgResearch-Ruakura, Hamilton, New Zealand
Interferon-tau (IFN-
) is secreted from trophectoderm of periimplantation ruminant conceptuses and is a critical component of pregnancy recognition. Multiple genes encode IFN-. The objectives of this study were to identify expressed forms of ovine IFN- and to compare their biological activities. Sequences analyzed after cloning 36 reverse transcription-polymerase chain reaction products of ovine conceptus RNA provided seven new cDNA that were similar in sequence to previously cloned forms (p3, p6, and p8 cDNA). Phylogenetic analysis of amino acid sequence for all new and previously reported forms showed that ovine IFN- forms can be divided into three main groups. Equivalent amounts of mRNA for p3, p6, and p8 forms were detected in conceptuses following RNase protection. Recombinant p3 and p8 protein had similar antiviral activity on ovine and bovine cells whereas p6 protein was less active. The p3 form was the most potent of the three in its ability to extend estrous cycle length in nonpregnant ewes. In summary, there appeared to be three main groups of ovine IFN-, each containing several variant forms. Antiviral activity was not particularly well correlated with ability to prevent luteolysis, suggesting that distinct intracellular mechanisms are used to exert the various actions of IFN-.
2 Correspondence: Alan D. Ealy, Department of Animal Sciences, 158 Animal Science Research Center, University of Missouri, Columbia, MO 65211–5300. FAX: 573 882 6827; ealya{at}missouri.edu
This article has been cited by other articles:
|
|
 |
|
  Y. Chen, J. A. Green, E. Antoniou, A. D. Ealy, N. Mathialagan, A. M. Walker, M. P. Avalle, C. S. Rosenfeld, L. B. Hearne, and R. M. Roberts Effect of Interferon-{tau} Administration on Endometrium of Nonpregnant Ewes: A Comparison with Pregnant Ewes Endocrinology, May 1, 2006; 147(5): 2127 - 2137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.P. Green, L.D. Spate, J.A. Bixby, A.D. Ealy, and R.M. Roberts A Comparison of the Anti-Luteolytic Activities of Recombinant Ovine Interferon-Alpha and -Tau in Sheep Biol Reprod, December 1, 2005; 73(6): 1087 - 1093. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-I. Kawamoto, K. Oritani, H. Asada, I. Takahashi, J. Ishikawa, H. Yoshida, M. Yamada, N. Ishida, H. Ujiie, H. Masaie, et al. Antiviral Activity of Limitin against Encephalomyocarditis Virus, Herpes Simplex Virus, and Mouse Hepatitis Virus: Diverse Requirements by Limitin and Alpha Interferon for Interferon Regulatory Factor 1 J. Virol., September 1, 2003; 77(17): 9622 - 9631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Parent, C. Villeneuve, A. P. Alexenko, A. D. Ealy, and M. A. Fortier Influence of Different Isoforms of Recombinant Trophoblastic Interferons on Prostaglandin Production in Cultured Bovine Endometrial Cells Biol Reprod, March 1, 2003; 68(3): 1035 - 1043. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. D. Ealy, S. F. Larson, L. Liu, A. P. Alexenko, G. L. Winkelman, H. M. Kubisch, J. A. Bixby, and R. M. Roberts Polymorphic Forms of Expressed Bovine Interferon-{{tau}} Genes: Relative Transcript Abundance during Early Placental Development, Promoter Sequences of Genes and Biological Activity of Protein Products Endocrinology, July 1, 2001; 142(7): 2906 - 2915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Swasdipan, H. Bielefeldt-Ohmann, N. Phillips, P. D. Kirkland, and M. R. McGowan Rapid Transplacental Infection with Bovine Pestivirus Following Intranasal Inoculation of Ewes in Early Pregnancy Vet. Pathol., May 1, 2001; 38(3): 275 - 280. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
