| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a Institute of Reproduction and Development, Monash University, Monash Medical Centre, Clayton, Victoria 3168, Australia
b Colorado Center for Reproductive Medicine, Englewood, Colorado 80110
This study was an investigation of the interaction of lactate on pyruvate and glucose metabolism in the early mouse embryo. Pyruvate uptake and metabolism by mouse embryos were significantly affected by increasing the lactate concentration in the culture medium. In contrast, glucose uptake was not affected by lactate in the culture medium. At the zygote stage, the percentage of pyruvate taken up and oxidized was significantly reduced in the presence of increasing lactate, while at the blastocyst stage, increasing the lactate concentration increased the percentage of pyruvate oxidized. Lactate oxidation was determined to be 3-fold higher (when lactate was present at 20 mM) at the blastocyst stage compared to the zygote. Analysis of the kinetics of lactate dehydrogenase (LDH) determined that while the Vmax of LDH was higher at the zygote stage, the Km of LDH was identical for both stages of development, confirming that the LDH isozyme was the same. Furthermore, the activity of LDH isolated from both stages was reduced by 40% in the presence of 20 mM lactate. The observed differences in lactate metabolism between the zygote and blastocyst must therefore be attributed to in situ regulation of LDH. Activity of isolated LDH was found to be affected by nicotinamide adenine dinucleotide+ (NAD+) concentration. In the presence of increasing concentrations of lactate, zygotes exhibited an increase in autofluorescence consistent with a depletion of NAD+ in the cytosol. No increase was observed for later-stage embryos. Therefore it is proposed that the differences in pyruvate and lactate metabolism at the different stages of development are due to differences in the in situ regulation of LDH by cytosolic redox potential.
1 M.L. was the recipient of a Dora Lush Biomedical Studentship from the Australian National Health and Medical Research Council. D.K.G. was the recipient of a Fellowship from the Australian Research Council.
2 Correspondence: Michelle Lane, Colorado Center for Reproductive Medicine, 799 East Hampden Ave., Suite 300, Englewood, CO 80110. FAX: 303 788 4438; mlane{at}colocrm.com
This article has been cited by other articles:
|
|
 |
|
  S. E Harris, I. Adriaens, H. J Leese, R. G Gosden, and H. M Picton Carbohydrate metabolism by murine ovarian follicles and oocytes grown in vitro Reproduction, September 1, 2007; 134(3): 415 - 424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. R. Herrick, J. B. Bond, G. M. Magarey, H. L. Bateman, R. L. Krisher, S. A. Dunford, and W. F. Swanson Toward a Feline-Optimized Culture Medium: Impact of Ions, Carbohydrates, Essential Amino Acids, Vitamins, and Serum on Development and Metabolism of In Vitro Fertilization-Derived Feline Embryos Relative to Embryos Grown In Vivo Biol Reprod, May 1, 2007; 76(5): 858 - 870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.S. Lopes, S.E. Madsen, N.B. Ramsing, P. Lovendahl, T. Greve, and H. Callesen Investigation of respiration of individual bovine embryos produced in vivo and in vitro and correlation with viability following transfer Hum. Reprod., February 1, 2007; 22(2): 558 - 566. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Dumollard, Z. Ward, J. Carroll, and M. R. Duchen Regulation of redox metabolism in the mouse oocyte and embryo Development, February 1, 2007; 134(3): 455 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Satrustegui, B. Pardo, and A. del Arco Mitochondrial Transporters as Novel Targets for Intracellular Calcium Signaling Physiol Rev, January 1, 2007; 87(1): 29 - 67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jansen, T. Esmaeilpour, M. Pantaleon, and P. L Kaye Glucose affects monocarboxylate cotransporter (MCT) 1 expression during mouse preimplantation development. Reproduction, March 1, 2006; 131(3): 469 - 479. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Zander, J. G. Thompson, and M. Lane Perturbations in Mouse Embryo Development and Viability Caused by Ammonium Are More Severe after Exposure at the Cleavage Stages Biol Reprod, February 1, 2006; 74(2): 288 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Lane and D. K. Gardner Mitochondrial Malate-Aspartate Shuttle Regulates Mouse Embryo Nutrient Consumption J. Biol. Chem., May 6, 2005; 280(18): 18361 - 18367. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Thouas, A. O. Trounson, E. J. Wolvetang, and G. M. Jones Mitochondrial Dysfunction in Mouse Oocytes Results in Preimplantation Embryo Arrest in Vitro Biol Reprod, December 1, 2004; 71(6): 1936 - 1942. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Herrick, P. Bartels, and R. L. Krisher Postthaw Evaluation of In Vitro Function of Epididymal Spermatozoa from Four Species of Free-Ranging African Bovids Biol Reprod, September 1, 2004; 71(3): 948 - 958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Dumollard, P. Marangos, G. Fitzharris, K. Swann, M. Duchen, and J. Carroll Sperm-triggered [Ca2+] oscillations and Ca2+ homeostasis in the mouse egg have an absolute requirement for mitochondrial ATP production Development, July 1, 2004; 131(13): 3057 - 3067. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lane and D. K. Gardner Ammonium Induces Aberrant Blastocyst Differentiation, Metabolism, pH Regulation, Gene Expression and Subsequently Alters Fetal Development in the Mouse Biol Reprod, October 1, 2003; 69(4): 1109 - 1117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lane, J. M. Maybach, and D. K. Gardner Addition of ascorbate during cryopreservation stimulates subsequent embryo development Hum. Reprod., October 1, 2002; 17(10): 2686 - 2693. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yoshioka, C. Suzuki, A. Tanaka, I. M.-K. Anas, and S. Iwamura Birth of Piglets Derived from Porcine Zygotes Cultured in a Chemically Defined Medium Biol Reprod, January 1, 2002; 66(1): 112 - 119. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
