HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Spaziani, E. P. Articles by Gould, S. F. Search for Related Content PubMed PubMed Citation Articles by Spaziani, E. P. Articles by Gould, S. F. Agricola Articles by Spaziani, E. P. Articles by Gould, S. F.

Corticotropin-Releasing Hormone Increases the Expression of the Prostaglandin E₂ Receptor Subtype EP1 in Amnion WISH Cells

^a Department of Obstetrics & Gynecology, University of South Florida Health Science Center, Tampa, Florida 33612

The purpose of this study was to investigate the effect of corticotropin-releasing hormone (CRH) on the expression of the prostaglandin (PG) E₂ EP1 receptor subtype and PGE₂ production in amnion WISH cells (AWC). AWC cultures were incubated with CRH. Culture fluid was collected for PGE₂ measurement, and the cells were collected and analyzed for EP1 protein and mRNA. Immunohistochemical localization of the EP1 receptor was also performed. Incubation of AWC with CRH resulted in a dose-dependent increase (r = 0.97) in the level of EP1 receptor protein (P < 0.001). Coincubation of AWC with CRH and indomethacin resulted in the decreased production of PGE₂ while having no effect on EP1 receptor expression. A significant but not dose-dependent increase in EP1 mRNA expression was also observed (P < 0.01). Immunohistochemical evaluation verified cell membrane localization of the receptor in both stimulated and unstimulated cells and confirmed the increased expression of EP1 receptor in response to CRH. Incubation of AWC with CRH also resulted in increased culture fluid PGE₂ levels (P < 0.01). These results suggest that the role CRH plays in the initiation of labor may also involve the promotion of elevated PGE₂ levels and increased expression of the EP1 receptor in amnion.

First decision: 17 June 1999.

¹ Correspondence: Eric P. Spaziani, University of South Florida Health Science Center, Department of Obstetrics & Gynecology, Box 18, 12901 Bruce B. Downs Boulevard, Tampa, FL 33612. FAX: 813 974 7026; espazian{at}com1.med.usf.edu

This article has been cited by other articles:

				R. Menon, C. P. Arora, C. J. Hobel, and S. J. Fortunato Corticotrophin-Releasing Hormone in Lipopolysaccharide-Stimulated Term Fetal Membranes and Amniotic Fluid From Term and Preterm Birth in African Americans and Caucasians Reproductive Sciences, May 1, 2008; 15(5): 477 - 483. [Abstract] [PDF]