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A Tumor Necrosis Factor Decoy Receptor Homologue Is Up-Regulated in the Brook Trout (Salvelinus fontinalis) Ovary at the Completion of Ovulation¹

^a Institut National de la Recherche Agronomique, S.C.R.I.B.E., Campus de Beaulieu, 35042 Rennes Cedex, France ^b University of Notre Dame, Department of Biological Sciences, Notre Dame, Indiana 46556

An up-regulated cDNA fragment was obtained from differential-display polymerase chain reaction of brook trout ovarian tissue stimulated by phorbol-12-myristate-13-acetate (PMA) and calcium ionophore A23187. Using this cDNA as a probe, a full-length cDNA of 2267 base pairs was obtained by screening a library of PMA/A23187-stimulated ovarian cDNA. The mRNA obtained presumably encodes for a 302-amino acid protein showing similarities with several members of the tumor necrosis factor (TNF) receptor superfamily. The protein contains several cysteine-rich domains characteristic of mammalian TNF receptor members and is most similar to human decoy receptor 3 and osteoprotegerin, two soluble decoy TNF receptors. Consequently, this TNF receptor homologue was tentatively named a trout decoy receptor (TDcR). On Northern blots of ovarian tissue, TDcR hybridized with a 2.2-kilobase transcript that was strongly up-regulated under phorbol ester stimulation. TDcR mRNA was localized in granulosa cells and was detected in the ovary during and after natural ovulation. Its expression was up-regulated at the end of ovulation and progressively down-regulated after 48 h postovulation. Among other trout tissues tested, the transcript was present only in the testis. To our knowledge this is the first description of a member of the TNF receptor family from a lower vertebrate and the first report of a decoy-like TNF receptor in the vertebrate ovary.

First decision: 10 August 1999.

¹ This study was supported by USDA grant #95-37203-1962.

² Correspondence: Frederick William Goetz, Department of Biological Sciences, University of Notre Dame, P.O. Box 369, Notre Dame, IN 46556-0369. FAX: 219 631 7413; goetz.1{at}nd.edu

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