| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a Department of Obstetrics and Gynecology, Oita Medical University, Oita 879-5593, Japan
Platelet-activating factor (PAF) is involved in such reproductive processes as parturition. We investigated the effect of PAF on the expression of matrix metalloproteinase-1 (MMP-1) and that of tissue inhibitor of metalloproteinases-1 (TIMP-1) in human uterine cervical fibroblasts. Uterine cervical tissue was obtained from patients who underwent cesarean section at term. Collagenase-dispersed fibroblasts were cultured and used in the experiments. PAF receptor was identified in the uterine cervical fibroblasts by use of reverse transcription-polymerase chain reaction and Southern blot analysis. Northern blot analysis showed that PAF increased the expression of MMP-1 mRNA in a time-dependent manner, whereas expression of TIMP-1 mRNA was not affected by PAF. Concentration of MMP-1 protein in the PAF-treated culture media significantly exceeded that in control cultures. The PAF-induced production of MMP-1 protein was abolished by treatment with WEB 2170, a specific PAF receptor antagonist. Results suggest that PAF may accelerate collagenolysis in the human uterine cervix by inducing an imbalance in the activity between MMP-1 and TIMP-1, thus contributing to the cervical ripening during parturition.
1 Correspondence: Terumasa Sugano, Department of Obstetrics and Gynecology, Oita Medical University, Hasama-machi, Oita 879-5593, Japan. FAX: 81 97 549 5087; sugano{at}oita-med.ac.jp
This article has been cited by other articles:
|
|
 |
|
  D. D. Deo, N. G. Bazan, and J. D. Hunt Activation of Platelet-activating Factor Receptor-coupled G{alpha}q Leads to Stimulation of Src and Focal Adhesion Kinase via Two Separate Pathways in Human Umbilical Vein Endothelial Cells J. Biol. Chem., January 30, 2004; 279(5): 3497 - 3508. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Narahara, Y. Kawano, K. Nasu, J. Yoshimatsu, J. M. Johnston, and I. Miyakawa Platelet-Activating Factor Inhibits the Secretion of Platelet-Activating Factor Acetylhydrolase by Human Decidual Macrophages J. Clin. Endocrinol. Metab., December 1, 2003; 88(12): 6029 - 6033. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Elovitz, Z. Wang, E. K. Chien, D. F. Rychlik, and M. Phillippe A New Model for Inflammation-Induced Preterm Birth: The Role of Platelet-Activating Factor and Toll-Like Receptor-4 Am. J. Pathol., November 1, 2003; 163(5): 2103 - 2111. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Yoshida, N. Sagawa, H. Itoh, S. Yura, M. Takemura, Y. Wada, T. Sato, A. Ito, and S. Fujii Prostaglandin F2{alpha}, cytokines and cyclic mechanical stretch augment matrix metalloproteinase-1 secretion from cultured human uterine cervical fibroblast cells Mol. Hum. Reprod., July 1, 2002; 8(7): 681 - 687. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.W. Marvin, J.A. Keelan, R.L. Eykholt, T.A. Sato, and M.D. Mitchell Use of cDNA arrays to generate differential expression profiles for inflammatory genes in human gestational membranes delivered at term and preterm Mol. Hum. Reprod., April 1, 2002; 8(4): 399 - 408. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Yoshida, N. Sagawa, H. Itoh, S. Yura, D. Korita, K. Kakui, N. Hirota, T. Sato, A. Ito, and S. Fujii Nitric oxide increases matrix metalloproteinase-1 production in human uterine cervical fibroblast cells Mol. Hum. Reprod., October 1, 2001; 7(10): 979 - 985. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Sugano, H. Narahara, K. Nasu, K. Arima, K. Fujisawa, and I. Miyakawa Effects of platelet-activating factor on cytokine production by human uterine cervical fibroblasts Mol. Hum. Reprod., May 1, 2001; 7(5): 475 - 481. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
