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a Department of Physiology, University of Adelaide, Adelaide, South Australia, Australia 5005
b Department of Physiology, College of Medicine, University of Oklahoma, Health Sciences Center, Oklahoma City, Oklahoma 73190
c Department of Physiology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853-6401
d Department of Obstetrics and Gynecology, Perinatal Research Laboratories, University of Wisconsin-Madison, Madison, Wisconsin 53715
In the present study we investigated the ontogeny of the expression of the type 1 angiotensin receptor (AT1R mRNA) and the zonal localization of AT1R immunoreactivity (AT1R-ir) and cytochrome P450c11 (CYP11B-ir) in the sheep adrenal gland. In the adult sheep and in the fetus from as early as 90 days gestation, intense AT1R-ir was observed predominantly in the zona glomerulosa and to a lesser extent in the zona fasciculata, and it was not detectable in the adrenal medulla. AT1R mRNA decreased 4-fold between 105 days and 120 days, whereas AT1R mRNA levels remained relatively constant between 120 days and the newborn period. In contrast, both in the adult sheep and in the fetal sheep from as early as 90 days gestation, intense CYP11B-ir was consistently detected throughout the adrenal cortex and in steroidogenic cells that surround the central adrenal vein. In conclusion, we speculate that the presence of AT1R in the zona fasciculata, and the higher levels of expression of AT1R at around 100 days gestation, may suggest that suppression of CYP17 is mediated via AT1R at this time. The abundant expression of AT1R-ir and CYP11B-ir in the zona glomerulosa of the fetal sheep adrenal gland would also suggest that lack of angiotensin II stimulation of aldosterone secretion is not due to an absence of AT1R or CYP11B in the zona glomerulosa.
1 The work was supported in part by NIH Grants HL56702 (I.M.B.) and HD21350 (P.W.N.) and by USDA 9601773 (I.M.B.), AHA-WI 95-GB-41 (I.M.B.). C.L.C. was supported by the NHMRC of Australia (990275) and a J.B. Reid Fellowship from The University of Adelaide, South Australia. Presented in part at the 8th Adrenal Cortex Conference, Orford, Quebec, 1998.
2 Correspondence: Catherine L. Coulter, Department of Physiology, Medical School Building, Room N411, The University of Adelaide, GPO Box 498, North Terrace, Adelaide, South Australia 5005, Australia. FAX: 61 8 8303 3356; catherine.coulter{at}adelaide.edu.au
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