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a INSERM U. 361, Université René Descartes Pavillon Baudelocque, 75014 Paris, France
In conclusion, we report here the first evidence of the presence of PR in the muscular layer of human term fetoplacental vessels. This finding, together with the high progesterone concentrations in cord blood, suggests that the interactions between the PR and its ligand may play a role in the physiology and physiopathology of human fetoplacental vascularization.
The presence of progesterone receptors (PR) throughout the human term fetoplacental vascular tree was investigated. By reverse transcription-polymerase chain reaction (RT-PCR), we showed expression of PR mRNAs in stem villi vessels, chorionic arteries and veins, and umbilical arteries and veins. Binding studies and Scatchard analysis revealed a single class of high-affinity binding sites for 3H-R5020 (promegestone) in cytosolic extracts of all placental vessels, with Kd values in the range of 2.5–4 nM. High levels of PR were detected in placental vessels compared to other vascular tissues. Thus, maximum binding capacities of stem villi vessels, chorionic arteries and veins, and umbilical arteries and veins were 247 ± 25, 377 ± 58, 295 ± 40, 371 ± 118, and 672 ± 144 fmol/mg protein, respectively. Endothelial cell elimination in chorionic arteries did not significantly modify the number of PR. RT-PCR and binding studies also assessed PR expression in cultured placental vascular smooth muscle cells isolated from stem villi vessels. All these data suggested that most of the PR of fetoplacental vessels were from the media.
1 Supported by Institut National de la Santé et de la Recherche Médicale and by University René-Descartes.
2 Correspondence: F. Ferré, INSERM U. 361, Pavillon Baudelocque, 123, Boulevard de Port-Royal, 75014 Paris, France. FAX: 33 143 26 44 08; u361{at}cochin.inserm.fr
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