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a MRC Group in Fetal and Neonatal Health and Development,
b Departments of Physiology and Obstetrics and Gynecology, University of Toronto, Toronto, Ontario, Canada M5S 1A8
c MRC Group in Development and Fetal Health,
d Samuel Lunenfeld Research Institute, Mount Sinai Hospital Toronto, Toronto, Ontario, Canada M5G 1X5
e Department of Cellular and Molecular Medicine and Obstetrics and Gynecology, University of Ottawa, Ottawa, Ontario, Canada K1H 8L6
We examined whether spontaneous parturition in sheep was associated with tissue-specific changes in prostaglandin H2 synthase-2 (PGHS-2) expression and/or with altered expression of myometrial EP and FP receptors. Placental and uterine tissues were collected from three groups of chronically catheterized sheep in relation to term spontaneous labor: late pregnancy, not in labor; early labor; and active labor. Expression of PGHS-2 mRNA and protein was determined by in situ hybridization, Western blotting, and immunohistochemistry. Semiquantitative reverse transcription-polymerase chain reaction was used to assess the presence of and changes in prostaglandin (PG) receptor subtypes. In placenta, PGHS-2 mRNA and protein localized to trophoblast uninucleate cells and tended to increase with early labor. PGHS-2 mRNA and protein localized to endometrial epithelium and to myometrium, where PGHS-2 protein levels rose in active labor tissues. Concentrations of PGE2 in fetal plasma rose progressively with labor, whereas 13,14-dihydro-15-keto-PGF2
in maternal plasma increased significantly only in active labor. Messenger RNA encoding four EP receptor subtypes and FP receptor were present in myometrium, but levels did not change with labor. We suggest that spontaneous labor in sheep is associated with a progressive increase in PGHS-2 expression in a temporal and tissue-specific manner from trophoblast to maternal tissues, rather than alteration in PG receptor gene expression.
1 This work was supported by the Medical Research Council of Canada (MT-14097).
2 Correspondence: Sandor Gyomorey, Department of Physiology, University of Toronto, 1 King's College Circle, Medical Sciences Bldg., Room 3344, Toronto, ON, Canada M5S 1A8. FAX: 416 978 4940; j.challis{at}utoronto.ca
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