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on Steroidogenic Components and Oligonucleosomes in Ovine Luteal Tissue1
a Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins, Colorado 80526
To determine whether prostaglandin (PG) F2
had a dose-dependent effect upon secretion of progesterone, oligonucleosome formation, or loss of luteal weight, ewes on Day 9 or 10 of the estrous cycle were administered 0, 3, 10, or 30 mg PGF2 per 60 kg BW (i.v.), and luteal tissue was collected 9 and 24 h after injection. All doses of PGF2 decreased (P < 0.05) concentrations of progesterone in sera by 9 h; however, in ewes treated with 3 mg PGF2, concentrations of progesterone were similar to control values at 24 h and higher (P < 0.05) than those in the 10- or 30-mg groups. Concentrations of progesterone in sera over all dose levels were highly correlated to luteal concentrations of mRNA encoding steroidogenic acute regulatory protein (P < 0.001), cytochrome P450 side-chain cleavage (P < 0.02), and 3ß-hydroxysteroid dehydrogenase (P < 0.01). Corpora lutea collected at 24 h from ewes treated with the 10- and 30-mg doses of PGF2 weighed less (P < 0.05) than those from controls. Oligonucleosomes were not present in luteal tissues from control ewes. Surprisingly, all doses of PGF2-induced oligonucleosomes in a majority of animals at 9 h and in a majority of ewes treated with 10 and 30 mg of PGF2 at 24 h. In conclusion, 3 mg of PGF2 per 60 kg BW transiently decreased serum concentrations of progesterone and induced oligonucleosome formation, but did not result in reduced luteal weight. The 10- and 30-mg doses of PGF2 decreased secretion of progesterone and induced oligonucleosome formation and luteolysis.
1 This research was supported by NIH grant HD 11590 and the Colorado Agricultural Experiment Station.
2 Correspondence. FAX: 970 491 3557; gniswend{at}cvmbs.colostate.edu
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