| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a Department of Zoology & Genetics, Iowa State University, Ames, Iowa 50011-3223
Fertilization-induced Ca2+ oscillations in mouse eggs cease at the time of pronuclear formation when maturation-promoting factor (MPF) is inactivated, but the Ca2+ oscillations are ceaseless if eggs are arrested at metaphase by colcemid, which maintains the activity of MPF. To determine the possible role of MPF in regulation of cytoplasmic Ca2+ excitability, roscovitine, a specific inhibitor of p34cdc2/cyclin B kinase, was used to inactivate MPF, and its effect on fertilization-induced Ca2+ oscillations was investigated. Our results showed that roscovitine at 
50 µM suppressed fertilization-induced Ca2+ oscillations in normal and colcemid-treated metaphase II (MII) eggs after the first 1–2 Ca2+ spikes. Roscovitine inhibition of fertilization-induced Ca2+ oscillations could be reversed by extensive washing of the eggs. Histone H1 kinase activity in colcemid-treated MII eggs was similarly inhibited by roscovitine, which suggested that the cessation of fertilization-induced Ca2+ oscillations is due to the inactivation of MPF. Thimerosal-induced Ca2+ oscillations in Ca2+-, Mg2+-free medium was also suppressed by roscovitine, suggesting a general inhibitory effect of roscovitine on Ca2+ oscillations. The inhibition may be achieved by disruption of Ca2+ release and refilling of the calcium store. Thapsigargin, an inhibitor of the endoplasmic reticulum Ca-ATPase, induced significantly less Ca2+ release in roscovitine-treated eggs than in the non-drug-treated eggs. Taken together, our results suggest that MPF plays an important role in regulation of the cytoplasmic Ca2+ excitability in mouse eggs.
1 This study was supported by NSF (IBN96-31982).
2 Correspondence: Man-qi Deng, University of Connecticut, Department of Animal Science/Biotech Center, 3636 Horsebarn Road, Extension U-40, Storrs, CT 06269-4040. FAX: 860 486 4375; mdeng{at}ansc1.cag.uconn.edu
This article has been cited by other articles:
|
|
 |
|
  M. Levasseur, M. Carroll, K. T. Jones, and A. McDougall A novel mechanism controls the Ca2+ oscillations triggered by activation of ascidian eggs and has an absolute requirement for Cdk1 activity J. Cell Sci., May 15, 2007; 120(10): 1763 - 1771. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Li, K. Malathi, O. Krizanova, K. Ondrias, K. Sperber, V. Ablamunits, and T. Jayaraman Cdc2/Cyclin B1 Interacts with and Modulates Inositol 1,4,5-Trisphosphate Receptor (Type 1) Functions J. Immunol., November 1, 2005; 175(9): 6205 - 6210. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-M. Hsu, Y.-C. G. Lee, C.-T. R. Yu, and C.-Y. F. Huang Fbx7 Functions in the SCF Complex Regulating Cdk1-Cyclin B-phosphorylated Hepatoma Up-regulated Protein (HURP) Proteolysis by a Proline-rich Region J. Biol. Chem., July 30, 2004; 279(31): 32592 - 32602. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Lim, E. Ercolano, K. Kyozuka, G. A. Nusco, F. Moccia, K. Lange, and L. Santella The M-phase-promoting Factor Modulates the Sensitivity of the Ca2+ Stores to Inositol 1,4,5-Trisphosphate via the Actin Cytoskeleton J. Biol. Chem., October 24, 2003; 278(43): 42505 - 42514. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Stricker and T. L. Smythe Endoplasmic reticulum reorganizations and Ca2+ signaling in maturing and fertilized oocytes of marine protostome worms: the roles of MAPKs and MPF Development, July 1, 2003; 130(13): 2867 - 2879. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Marangos, G. FitzHarris, and J. Carroll Ca2+ oscillations at fertilization in mammals are regulated by the formation of pronuclei Development, April 1, 2003; 130(7): 1461 - 1472. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. FitzHarris, P. Marangos, and J. Carroll Cell Cycle-dependent Regulation of Structure of Endoplasmic Reticulum and Inositol 1,4,5-Trisphosphate-induced Ca2+ Release in Mouse Oocytes and Embryos Mol. Biol. Cell, January 1, 2003; 14(1): 288 - 301. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. C. Gordo, M. Kurokawa, H. Wu, and R. A. Fissore Modifications of the Ca2+ release mechanisms of mouse oocytes by fertilization and by sperm factor Mol. Hum. Reprod., July 1, 2002; 8(7): 619 - 629. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Schang, A. Bantly, and P. A. Schaffer Explant-Induced Reactivation of Herpes Simplex Virus Occurs in Neurons Expressing Nuclear cdk2 and cdk4 J. Virol., June 27, 2002; 76(15): 7724 - 7735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Allard, M. J. Champigny, S. Skoggard, J. A. Erkmann, M. L. Whitfield, W. F. Marzluff, and H. J. Clarke Stem-loop binding protein accumulates during oocyte maturation and is not cell-cycle-regulated in the early mouse embryo J. Cell Sci., January 12, 2002; 115(23): 4577 - 4586. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. LIM, K. KYOZUKA, G. GRAGNANIELLO, E. CARAFOLI, and L. SANTELLA NAADP+ initiates the Ca2+ response during fertilization of starfish oocytes FASEB J, October 1, 2001; 15(12): 2257 - 2267. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
