| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China
Matrix metalloproteinases (MMPs) are implicated in the degradation of extracellular matrix; they play important roles in the invasion of the trophoblast cell into the maternal endometrium during placentation. Previous studies have concentrated on comparison of MMP expression in trophoblast cells between the first and third trimester. But the dynamic expression of MMPs during the first trimester has not been reported. In the present study, the expression of MMP-2, -9, and -14 (membrane-type MMP-1) and the production of tissue inhibitors of metalloproteinases (TIMP-1 and TIMP-2) by cultured human cytotrophoblast cells from 6 to 11 wk of gestation were investigated. The cells were cultured under serum-free conditions. There was no MMP-9 secretion by the cells at Week 6, but from Week 7 to 11 the MMP-9 secretion increased gradually. Week 11 cells secreted more than 10-fold as much MMP-9 (167.7 ± 18.8 ng/ml) as Week 7 (14.7 ± 3.9 ng/ml) cultures. However, MMP-2 production declined from Week 6 to Week 11, and the production at Week 11 (32.3 ± 8.1 ng/ml) was about one sixth that at Week 6 (205.7 ± 27.2 ng/ml). The expression of mRNA transcripts for MMP-2 and MMP-9 correlated with enzyme secretion; we did not detect any MMP-9 mRNA signal in 20 µg total RNA extracted from cultured cells at Weeks 6, 7, and 8 of pregnancy, but a signal was apparent in Weeks 9 and 11. MMP-2 mRNA was expressed throughout the 6- to 11-wk period and exhibited a remarkable decline during this period. MMP-14 mRNA transcripts remained relatively stable from 6 to 11 wk. Significantly more TIMP-1 (P < 0.01) was detected in Week 9 (87.5 ± 15.0 ng/ml) and Week 11 (169.1 ± 30.2 ng/ml) media compared to Week 6 media (23.5 ± 4.8 ng/ml), but we did not detect any TIMP-2 in the media of the tested cells. This study demonstrated that first-trimester human cytotrophoblast cells were able to produce abundant laminin, fibronectin, and vitronectin. However, we did not observe detectable secretion of collagen I and collagen IV. These data indicated that human trophoblast-derived MMPs and their inhibitors are intrinsically and developmentally regulated. The same cytotrophoblast cells that produced MMPs could also secrete various substrates for these enzymes.
1 This study was supported by the ninety-fifth National Pan-Deng project, the Knowledge Innovation Program from the Chinese Academy of Sciences, the grant 39330130# from National Natural Science Foundation of China, and the grant RF 94025 #30 from the Rockefeller Foundation.
2 Correspondence: Lin-zhi Zhuang, State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 19 Zhongguancun Lu, Beijing, 100080, China. FAX: 86 10 62565689; zhuanglz{at}panda.ioz.ac.cn
This article has been cited by other articles:
|
|
 |
|
  M. Yoshie, H. Kashima, T. Bessho, M. Takeichi, K. Isaka, and K. Tamura Expression of stathmin, a microtubule regulatory protein, is associated with the migration and differentiation of cultured early trophoblasts Hum. Reprod., August 20, 2008; (2008) den317v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Nelson and I. A. Greer The potential role of heparin in assisted conception Hum. Reprod. Update, August 12, 2008; (2008) dmn031v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cohen, C. Wuillemin, O. Irion, and P. Bischof Regulation of MMP-9 by p53 in first trimester cytotrophoblastic cells Hum. Reprod., July 16, 2008; (2008) den264v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Prast, L. Saleh, H. Husslein, S. Sonderegger, H. Helmer, and M. Knofler Human Chorionic Gonadotropin Stimulates Trophoblast Invasion through Extracellularly Regulated Kinase and AKT Signaling Endocrinology, March 1, 2008; 149(3): 979 - 987. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. V. Cockle, N. Gopichandran, J. J. Walker, M. I. Levene, and N. M. Orsi Matrix Metalloproteinases and Their Tissue Inhibitors in Preterm Perinatal Complications Reproductive Sciences, October 1, 2007; 14(7): 629 - 645. [Abstract] [PDF]
|
|
|
|
 |
|
 T. Ishikawa, T. Harada, T. Kubota, and T. Aso Testosterone inhibits matrix metalloproteinase-1 production in human endometrial stromal cells in vitro Reproduction, June 1, 2007; 133(6): 1233 - 1239. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-l. Wang, W. Qiu, H.-c. Feng, Y.-x. Li, L.-z. Zhuang, Z. Wang, Y. Liu, J.-q. Zhou, D.-h. Zhang, and G. S.W. Tsao Immortalization of normal human cytotrophoblast cells by reconstitution of telomeric reverse transcriptase activity Mol. Hum. Reprod., July 1, 2006; 12(7): 451 - 460. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Harun, L. Ruban, M. Matin, J. Draper, N.M. Jenkins, G.C. Liew, P.W. Andrews, T.C. Li, S.M. Laird, and H.D.M. Moore Cytotrophoblast stem cell lines derived from human embryonic stem cells and their capacity to mimic invasive implantation events Hum. Reprod., June 1, 2006; 21(6): 1349 - 1358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Cohen, A. Meisser, L. Haenggeli, and P. Bischof Involvement of MAPK pathway in TNF-{alpha}-induced MMP-9 expression in human trophoblastic cells Mol. Hum. Reprod., April 1, 2006; 12(4): 225 - 232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Goldman and E. Shalev Difference in Progesterone-Receptor Isoforms Ratio Between Early and Late First-Trimester Human Trophoblast Is Associated with Differential Cell Invasion and Matrix Metalloproteinase 2 Expression Biol Reprod, January 1, 2006; 74(1): 13 - 22. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mahameed, S. Goldman, D. Gabarin, A. Weiss, and E. Shalev The Effect of Serum From Women With Preeclampsia on JAR (trophoblast-like) Cell Line Reproductive Sciences, September 1, 2005; 12(6): e45 - e50. [Abstract] [PDF]
|
|
|
|
|
|
S X Bai, Y L Wang, L Qin, Z J Xiao, R Herva, and Y S Piao Dynamic expression of matrix metalloproteinases (MMP-2, -9 and -14) and the tissue inhibitors of MMPs (TIMP-1, -2 and -3) at the implantation site during tubal pregnancy Reproduction, January 1, 2005; 129(1): 103 - 113. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Qian, H.-Y. Lin, H.-M. Wang, X. Zhang, D.-L. Liu, Q.-L. Li, and C. Zhu Normoxic Induction of the Hypoxic-Inducible Factor-1{alpha} by Interleukin-1{beta} Involves the Extracellular Signal-Regulated Kinase 1/2 Pathway in Normal Human Cytotrophoblast Cells Biol Reprod, June 1, 2004; 70(6): 1822 - 1827. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-S. Chou, H. Zhu, C. D. MacCalman, and P. C. K. Leung Regulatory Effects of Gonadotropin-Releasing Hormone (GnRH) I and GnRH II on the Levels of Matrix Metalloproteinase (MMP)-2, MMP-9, and Tissue Inhibitor of Metalloproteinases-1 in Primary Cultures of Human Extravillous Cytotrophoblasts J. Clin. Endocrinol. Metab., October 1, 2003; 88(10): 4781 - 4790. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. Curry Jr. and K. G. Osteen The Matrix Metalloproteinase System: Changes, Regulation, and Impact throughout the Ovarian and Uterine Reproductive Cycle Endocr. Rev., August 1, 2003; 24(4): 428 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Baumgart, S.V. Lenk, S.A. Loening, and K. Jung Quantitative differences in matrix metalloproteinase (MMP)-2, but not in MMP-9, tissue inhibitor of metalloproteinase (TIMP)-1 or TIMP-2, in seminal plasma of normozoospermic and azoospermic patients Hum. Reprod., November 1, 2002; 17(11): 2919 - 2923. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zhang, Y.-J. Cao, Y.-G. Zhao, Q.-X. A. Sang, and E.-K. Duan Expression of matrix metalloproteinase-26 and tissue inhibitor of metalloproteinase-4 in human normal cytotrophoblast cells and a choriocarcinoma cell line, JEG-3 Mol. Hum. Reprod., July 1, 2002; 8(7): 659 - 666. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, Q. Li, L. Shao, and C. Zhu Expression of Matrix Metalloproteinase-2, -9, -14, and Tissue Inhibitors of Metalloproteinase-1, -2, -3 in the Endometrium and Placenta of Rhesus Monkey (Macaca mulatta) During Early Pregnancy Biol Reprod, July 1, 2001; 65(1): 31 - 40. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Xu, Y.-l. Wang, Y.-s. Piao, S.-x. Bai, Z.-j. Xiao, Y.-l. Jia, S.-y. Luo, and L.-z. Zhuang Effects of Matrix Proteins on the Expression of Matrix Metalloproteinase-2, -9, and -14 and Tissue Inhibitors of Metalloproteinases in Human Cytotrophoblast Cells During the First Trimester Biol Reprod, July 1, 2001; 65(1): 240 - 246. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Curry Jr and K. G. Osteen Cyclic Changes in the Matrix Metalloproteinase System in the Ovary and Uterus Biol Reprod, May 1, 2001; 64(5): 1285 - 1296. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
