| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a Department of Veterinary Physiology and Pharmacology,
b Department of Large Animal Medicine and Surgery, Texas A&M University, College Station, Texas 77843
c Ultimate Genetics, Franklin, Texas 77856
Day 30 pregnancy rates were similar for blastocysts derived from adult (6 of 26; 23%) or fetal (5 of 32; 16%) cells. Day 90 pregnancy rates were 3 of 26 for adult and 0 of 32 for the fetal cell lines. One viable bull calf derived from a 21-yr-old serum-starved adult skin fibroblast was born in August 1999. In summary, somatic NT embryo development rates were similar whether adult or fetal cells, from the same genotype, were used as donor cells. Serum starvation of these adult donor cells did not improve development rates of NT embryos to blastocyst, but when fetal cells were serum-starved, there was a significant increase in development to blastocyst.
This study compared the nuclear transfer (NT) embryo development rates of adult and fetal cells within the same genotype. The adult fibroblast cells were obtained from a 21-yr-old Brahman bull. The fetal cells were derived from a Day 40 NT fetus previously cloned using cells from the Brahman bull. Overall, similar numbers of blastocysts developed from both adult (53 of 190; 28%) and fetal (39 of 140; 28%) donor cells. Improved blastocyst development rates were observed when fetal cells were serum-starved (serum-fed 12% vs. serum-starved 43%; P < 0.01) whereas there was no similar benefit when adult cells were serum-starved (both serum-fed and serum-starved 28%).
1 This research was supported by grants from the Department of Large Animal Medicine and Surgery to J.R.H. and the Texas Coordinating Board of Higher Education, Advanced Technology Program to M.E.W.
2 Correspondence: Jonathan Hill, Box 34 Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853-6401. FAX: 607 253 3531; jrh35{at}cornell.edu
This article has been cited by other articles:
|
|
 |
|
  A. Caraty and D. C. Skinner Gonadotropin-Releasing Hormone in Third Ventricular Cerebrospinal Fluid: Endogenous Distribution and Exogenous Uptake Endocrinology, October 1, 2008; 149(10): 5227 - 5234. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. I Alexopoulos, P. Maddox-Hyttel, P. Tveden-Nyborg, N. T D'Cruz, T. R Tecirlioglu, M. A Cooney, K. Schauser, M. K Holland, and A. J French Developmental disparity between in vitro-produced and somatic cell nuclear transfer bovine days 14 and 21 embryos: implications for embryonic loss Reproduction, October 1, 2008; 136(4): 433 - 445. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Shi, F. Lu, Y. Wei, K. Cui, S. Yang, J. Wei, and Q. Liu Buffalos (Bubalus bubalis) Cloned by Nuclear Transfer of Somatic Cells Biol Reprod, August 1, 2007; 77(2): 285 - 291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Wuensch, F. A. Habermann, S. Kurosaka, R. Klose, V. Zakhartchenko, H.-D. Reichenbach, F. Sinowatz, K. J. McLaughlin, and E. Wolf Quantitative Monitoring of Pluripotency Gene Activation after Somatic Cloning in Cattle Biol Reprod, June 1, 2007; 76(6): 983 - 991. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Westhusin, T. Shin, J. W. Templeton, R. C. Burghardt, and L. G. Adams Rescuing valuable genomes by animal cloning: A case for natural disease resistance in cattle J Anim Sci, January 1, 2007; 85(1): 138 - 142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R Arnold, V. Bordignon, R. Lefebvre, B. D Murphy, and L. C Smith Somatic cell nuclear transfer alters peri-implantation trophoblast differentiation in bovine embryos. Reproduction, August 1, 2006; 132(2): 279 - 290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K Hinrichs, Y H Choi, C C Love, Y G Chung, and D D Varner Production of horse foals via direct injection of roscovitine-treated donor cells and activation by injection of sperm extract. Reproduction, June 1, 2006; 131(6): 1063 - 1072. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Golding, C. R. Long, M. A. Carmell, G. J. Hannon, and M. E. Westhusin Suppression of prion protein in livestock by RNA interference PNAS, April 4, 2006; 103(14): 5285 - 5290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pfister-Genskow, C. Myers, L. A. Childs, J. C. Lacson, T. Patterson, J. M. Betthauser, P. J. Goueleke, R. W. Koppang, G. Lange, P. Fisher, et al. Identification of Differentially Expressed Genes in Individual Bovine Preimplantation Embryos Produced by Nuclear Transfer: Improper Reprogramming of Genes Required for Development Biol Reprod, March 1, 2005; 72(3): 546 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. V. Dindot, P. W. Farin, C. E. Farin, J. Romano, S. Walker, C. Long, and J. A. Piedrahita Epigenetic and Genomic Imprinting Analysis in Nuclear Transfer Derived Bos gaurus/Bos taurus Hybrid Fetuses Biol Reprod, August 1, 2004; 71(2): 470 - 478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.M. Powell, N.C. Talbot, K.D. Wells, D.E. Kerr, V.G. Pursel, and R.J. Wall Cell Donor Influences Success of Producing Cattle by Somatic Cell Nuclear Transfer Biol Reprod, July 1, 2004; 71(1): 210 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Farin, P. W. Farin, and J. A. Piedrahita Development of fetuses from in vitro-produced and cloned bovine embryos J Anim Sci, January 1, 2004; 82(13_suppl): E53 - 62. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Scanlan, L. Dufourny, and D. C. Skinner Somatostatin-14 Neurons in the Ovine Hypothalamus: Colocalization with Estrogen Receptor {alpha} and Somatostatin-28(1-12) Immunoreactivity, and Activation in Response to Estradiol Biol Reprod, October 1, 2003; 69(4): 1318 - 1324. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Inoue, N. Ogonuki, K. Mochida, Y. Yamamoto, K. Takano, T. Kohda, F. Ishino, and A. Ogura Effects of Donor Cell Type and Genotype on the Efficiency of Mouse Somatic Cell Cloning Biol Reprod, October 1, 2003; 69(4): 1394 - 1400. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.H. Choi, C.C. Love, Y.G. Chung, D.D. Varner, M.E. Westhusin, R.C. Burghardt, and K. Hinrichs Production of Nuclear Transfer Horse Embryos by Piezo-Driven Injection of Somatic Cell Nuclei and Activation with Stallion Sperm Cytosolic Extract Biol Reprod, August 1, 2002; 67(2): 561 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Hill, D. H. Schlafer, P. J. Fisher, and C. J. Davies Abnormal Expression of Trophoblast Major Histocompatibility Complex Class I Antigens in Cloned Bovine Pregnancies Is Associated with a Pronounced Endometrial Lymphocytic Response Biol Reprod, July 1, 2002; 67(1): 55 - 63. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Arat, J. Gibbons, S. J. Rzucidlo, D. S. Respess, M. Tumlin, and S. L. Stice In Vitro Development of Bovine Nuclear Transfer Embryos from Transgenic Clonal Lines of Adult and Fetal Fibroblast Cells of the Same Genotype Biol Reprod, June 1, 2002; 66(6): 1768 - 1774. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Govoni, X. C. Tian, G. W. Kazmer, M. Taneja, B. P. Enright, A. L. Rivard, X. Yang, and S. A. Zinn Age-Related Changes of the Somatotropic Axis in Cloned Holstein Calves Biol Reprod, May 1, 2002; 66(5): 1293 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-L. Liu, L.-Y. Sung, M. Barber, and X. Yang Hypertonic Medium Treatment for Localization of Nuclear Material in Bovine Metaphase II Oocytes Biol Reprod, May 1, 2002; 66(5): 1342 - 1349. [Abstract] [Full Text]
|
|
|
|
|
|
J. Gibbons, S. Arat, J. Rzucidlo, K. Miyoshi, R. Waltenburg, D. Respess, A. Venable, and S. Stice Enhanced Survivability of Cloned Calves Derived from Roscovitine-Treated Adult Somatic Cells Biol Reprod, April 1, 2002; 66(4): 895 - 900. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. C. Reggio, A. N. James, H. L. Green, W. G. Gavin, E. Behboodi, Y. Echelard, and R. A. Godke Cloned Transgenic Offspring Resulting from Somatic Cell Nuclear Transfer in the Goat: Oocytes Derived from Both Follicle-Stimulating Hormone-Stimulated and Nonstimulated Abattoir-Derived Ovaries Biol Reprod, November 1, 2001; 65(5): 1528 - 1533. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. M. Rideout III, K. Eggan, and R. Jaenisch Nuclear Cloning and Epigenetic Reprogramming of the Genome Science, August 10, 2001; 293(5532): 1093 - 1098. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Betts, V. Bordignon, J. R. Hill, Q. Winger, M. E. Westhusin, L. C. Smith, and W. A. King Reprogramming of telomerase activity and rebuilding of telomere length in cloned cattle PNAS, January 10, 2001; (2001) 31559298. [Abstract] [Full Text]
|
|
|
|
|
|
D. H. Betts, V. Bordignon, J. R. Hill, Q. Winger, M. E. Westhusin, L. C. Smith, and W. A. King Reprogramming of telomerase activity and rebuilding of telomere length in cloned cattle PNAS, January 30, 2001; 98(3): 1077 - 1082. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
