HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Simmons, D.G. Articles by Kennedy, T.G. Search for Related Content PubMed PubMed Citation Articles by Simmons, D.G. Articles by Kennedy, T.G. Agricola Articles by Simmons, D.G. Articles by Kennedy, T.G.

Induction of Glucose-Regulated Protein 78 in Rat Uterine Glandular Epithelium During Uterine Sensitization for the Decidual Cell Reaction¹

^a Departments of Physiology and Obstetrics and Gynaecology, The University of Western Ontario, London, Ontario, Canada N6A 5C1

Endometrial receptivity for implantation and sensitization for decidualization in rodents is a transient state under the control of the ovarian steroids estrogen and progesterone. It is unclear, however, what molecular events mediate the onset of uterine receptivity. Messenger RNA differential display was performed on endometrial RNA from ovariectomized rats differentially sensitized for decidualization. Maximally sensitized uteri were at the equivalent of Day 5 of pseudopregnancy, and temporally nonsensitized uteri at Day 4 or 6; hormonally nonsensitized uteri were from animals on Day 5 treated with low or high doses of estradiol on Day 4. A cDNA with endometrial expression restricted to maximally sensitized uteri was isolated, cloned, and sequenced. The cDNA matched the sequence for glucose-regulated protein 78 (GRP78), a heat shock 70-related protein that resides in the lumen of the endoplasmic reticulum (ER) and has roles in several cellular processes including multimeric protein assembly, the degradation of proteins, and the storage and regulation of ER luminal calcium. Northern blot analysis indicated a dramatic increase in GRP78 mRNA levels restricted to the sensitized, Day 5 endometrium, suggesting a role in the onset of the sensitized phase. In situ hybridization and immunohistochemistry experiments localized the up-regulation of GRP78 within the receptive endometrium to the glandular epithelium.

First decision: 13 October 1999.

¹ This work was supported by the Medical Research Council of Canada, Grant MT-10414.

² Correspondence: T.G. Kennedy, Department of Physiology, The University of Western Ontario, London, ON, Canada N6A 5C1. FAX: 519 661 3827; tom.kennedy{at}physiology.uwo.ca

This article has been cited by other articles:

				T. Parmar, S. Nimbkar-Joshi, R.R. Katkam, S. Gadkar-Sable, U. Chaudhari, D.D. Manjramkar, L. Savardekar, S. Jacob, C.P. Puri, and G. Sachdeva Differential expression of calreticulin, a reticuloplasmin in primate endometrium Hum. Reprod., May 21, 2009; (2009) dep187v1. [Abstract] [Full Text] [PDF]

				S. Ray, F. Xu, P. Li, N. S. Sanchez, H. Wang, and S. K. Das Increased Level of Cellular Bip Critically Determines Estrogenic Potency for a Xenoestrogen Kepone in the Mouse Uterus Endocrinology, October 1, 2007; 148(10): 4774 - 4785. [Abstract] [Full Text] [PDF]

				S. Ray, X. Hou, H.-E. Zhou, H. Wang, and S. K. Das Bip Is a Molecular Link between the Phase I and Phase II Estrogenic Responses in Uterus Mol. Endocrinol., August 1, 2006; 20(8): 1825 - 1837. [Abstract] [Full Text] [PDF]

				S. K. Dey, H. Lim, S. K. Das, J. Reese, B. C. Paria, T. Daikoku, and H. Wang Molecular Cues to Implantation Endocr. Rev., June 1, 2004; 25(3): 341 - 373. [Abstract] [Full Text] [PDF]

				D. G Simmons and T. G Kennedy Rat endometrial Vdup1 expression: changes related to sensitization for the decidual cell reaction and hormonal control Reproduction, April 1, 2004; 127(4): 475 - 482. [Abstract] [Full Text] [PDF]

				D. G. Simmons and T. G. Kennedy Uterine Sensitization-Associated Gene-1: A Novel Gene Induced Within the Rat Endometrium at the Time of Uterine Receptivity/Sensitization for the Decidual Cell Reaction Biol Reprod, November 1, 2002; 67(5): 1638 - 1645. [Abstract] [Full Text] [PDF]

				B. M. Bany and G. A. Schultz Increased Expression of a Novel Heat Shock Protein Transcript in the Mouse Uterus During Decidualization and in Response to Progesterone Biol Reprod, January 1, 2001; 64(1): 284 - 292. [Abstract] [Full Text]

				S. K. Das, J. Tan, S. Raja, J. Halder, B. C. Paria, and S. K. Dey Estrogen Targets Genes Involved in Protein Processing, Calcium Homeostasis, and Wnt Signaling in the Mouse Uterus Independent of Estrogen Receptor-alpha and -beta J. Biol. Chem., September 8, 2000; 275(37): 28834 - 28842. [Abstract] [Full Text] [PDF]