HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Xue, J.-C. Articles by Goldberg, E. Search for Related Content PubMed PubMed Citation Articles by Xue, J.-C. Articles by Goldberg, E. Agricola Articles by Xue, J.-C. Articles by Goldberg, E.

Identification of a Novel Testis-Specific Leucine-Rich Protein in Humans and Mice¹

^a Department of Biochemistry, Molecular Biology & Cell Biology, Northwestern University, Evanston, Illinois 60208

A novel testis-specific protein, termed LRTP, was identified by screening both human and mouse testis and mouse pachytene spermatocyte cDNA libraries. Sequence analyses (GenBank accession number: AF092208) revealed that LRTP contains an amino terminus leucine-rich repeat domain. There are several acidic regions rich in glutamic acid in the C-terminus. The sequence, by GenBank search, shows similarities to LANP and SDS22+, leucine-rich repeat proteins localized to the nucleus and involved in the regulation of protein phosphatases. In mouse, the mRNA is first detected at about Day 14 postpartum, presumably when mid-pachytene spermatocytes are first seen. In situ hybridization confirmed the expression of the LRTP mRNA at this stage of spermatogenesis. Immunohistochemical analysis revealed that the protein is most abundant in the cytoplasm of pachytene and diplotene cells, corresponding to late prophase of meiosis I. Immunohistochemical localization is markedly reduced in secondary spermatocytes, suggesting a functional association of LRTP with meiosis. An LRTP cDNA probe did not bind to mouse ovary RNA in a dot blot assay.

First decision: 11 October 1999.

¹ This work was supported by NICHD Sub-5-U54-HD29099 and by P30 HD28048.

² Correspondence: Erwin Goldberg, Department of Biochemistry, Molecular Biology & Cell Biology, Northwestern University, 2153 North Campus Drive, Evanston, IL 60208. FAX: 847 467 1380; erv{at}nwu.edu

This article has been cited by other articles:

				L. Dan, Y. Lifang, and L. Guangxiu Expression and Possible Functions of a Novel Gene SPATA12 in Human Testis J Androl, July 1, 2007; 28(4): 502 - 512. [Abstract] [Full Text] [PDF]

				G. W. Morgan, P. W. Denny, S. Vaughan, D. Goulding, T. R. Jeffries, D. F. Smith, K. Gull, and M. C. Field An Evolutionarily Conserved Coiled-Coil Protein Implicated in Polycystic Kidney Disease Is Involved in Basal Body Duplication and Flagellar Biogenesis in Trypanosoma brucei Mol. Cell. Biol., May 1, 2005; 25(9): 3774 - 3783. [Abstract] [Full Text] [PDF]

				J. Wu, F. Iwata, J. A. Grass, C. S. Osborne, L. Elnitski, P. Fraser, O. Ohneda, M. Yamamoto, and E. H. Bresnick Molecular Determinants of NOTCH4 Transcription in Vascular Endothelium Mol. Cell. Biol., February 15, 2005; 25(4): 1458 - 1474. [Abstract] [Full Text] [PDF]

				P. Padma, Y. Satouh, K.-i. Wakabayashi, A. Hozumi, Y. Ushimaru, R. Kamiya, and K. Inaba Identification of a Novel Leucine-rich Repeat Protein as a Component of Flagellar Radial Spoke in the Ascidian Ciona intestinalis Mol. Biol. Cell, February 1, 2003; 14(2): 774 - 785. [Abstract] [Full Text] [PDF]