| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Article |
vß3 Integrin Adversely Affects Implantation in the Mouse1
a Department of Physiology, School of Veterinary Medicine, Universidad Complutense, 28040 Madrid, Spain
b Lexicon Genetics, Inc., The Woodlands, Texas 77381
c Department of Obstetrics and Gynecology, Division of Reproductive Endocrinology, University of North Carolina, Chapel Hill, North Carolina 27599
The role of endometrial and embryonic integrins during implantation remains unresolved although work in animal models and in humans supports their involvement in this process. Temporal and spatial distribution of the 
vß3 integrin on both embryo and endometrium in women and mice coincides with the time of initial attachment during implantation. In mice, the endometrial and embryonic vß3 integrin is present at the time of implantation, as shown by reverse transcription-polymerase chain reaction and immunohistochemistry. In situ hybridization demonstrates the presence of the vß3 integrin on the subluminal stromal cells of the uterus. Functional blockade of this integrin on the day of implantation by intrauterine injection of neutralizing monoclonal antibodies against v or ß3 integrin subunits, arg-gly-asp (RGD)-containing peptides, or of the disintegrin echistatin, reduced the number of implantation sites compared to controls receiving BSA. These studies demonstrate that, like the human, the murine vß3 integrin is expressed at the time of implantation in the endometrium and on the blastocyst, and may play a critical role in the cascade of events leading to successful implantation.
1 Financial support provided by National Institutes of Health grants HD 35041 and HD-34824–1 (B.A.L.). This work was supported in part by the National Cooperative Program on Markers of Uterine Receptivity and the Fogerty International Fellowship Program (Y.G.). Sabbatical stay supported by Subprograma general de estancias en el extrajero grant from the Spanish Governnment, Ministry of Science and Education, Madrid, Spain.
2 Correspondence: Bruce A. Lessey, CB #7570 MacNider Bldg., University of North, Carolina, Chapel Hill, NC 27599. FAX: 919 966 5214; lessey{at}med.unc.edu
This article has been cited by other articles:
|
|
 |
|
  R. C Burghardt, J. R Burghardt, J. D Taylor II, A. T Reeder, B. T Nguen, T. E Spencer, K. J Bayless, and G. A Johnson Enhanced focal adhesion assembly reflects increased mechanosensation and mechanotransduction at maternal-conceptus interface and uterine wall during ovine pregnancy Reproduction, March 1, 2009; 137(3): 567 - 582. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J White, R. C Burghardt, J. Hu, M. M Joyce, T. E Spencer, and G. A Johnson Secreted phosphoprotein 1 (osteopontin) is expressed by stromal macrophages in cyclic and pregnant endometrium of mice, but is induced by estrogen in luminal epithelium during conceptus attachment for implantation. Reproduction, December 1, 2006; 132(6): 919 - 929. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-C. Ruan, X.-M. Zhu, Q. Luo, A.-X. Liu, Y.-L. Qian, C.-Y. Zhou, F. Jin, H.-F. Huang, and J.-Z. Sheng Ovarian stimulation with GnRH agonist, but not GnRH antagonist, partially restores the expression of endometrial integrin beta3 and leukaemia-inhibitory factor and improves uterine receptivity in mice Hum. Reprod., October 1, 2006; 21(10): 2521 - 2529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. S. Daftary and H. S. Taylor Endocrine Regulation of HOX Genes Endocr. Rev., June 1, 2006; 27(4): 331 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. P. Ramos, B. R. Rueda, P. C. Leavis, and R. R. Gonzalez Leptin Serves as an Upstream Activator of an Obligatory Signaling Cascade in the Embryo-Implantation Process Endocrinology, February 1, 2005; 146(2): 694 - 701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E Spencer, G. A Johnson, F. W Bazer, and R. C Burghardt Implantation mechanisms: insights from the sheep Reproduction, December 1, 2004; 128(6): 657 - 668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Y Lee and F. J DeMayo Animal models of implantation Reproduction, December 1, 2004; 128(6): 679 - 695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Dey, H. Lim, S. K. Das, J. Reese, B. C. Paria, T. Daikoku, and H. Wang Molecular Cues to Implantation Endocr. Rev., June 1, 2004; 25(3): 341 - 373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. I. Rodriguez, J.-G. Cheng, L. Liu, and C. L. Stewart Cochlin, a Secreted von Willebrand Factor Type A Domain-Containing Factor, Is Regulated by Leukemia Inhibitory Factor in the Uterus at the Time of Embryo Implantation Endocrinology, March 1, 2004; 145(3): 1410 - 1418. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Johnson, R. C. Burghardt, F. W. Bazer, and T. E. Spencer Osteopontin: Roles in Implantation and Placentation Biol Reprod, November 1, 2003; 69(5): 1458 - 1471. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Nagaoka, H. Nojima, F. Watanabe, K.-T. Chang, R. K. Christenson, S. Sakai, and K. Imakawa Regulation of Blastocyst Migration, Apposition, and Initial Adhesion by a Chemokine, Interferon {gamma}-inducible Protein 10 kDa (IP-10), during Early Gestation J. Biol. Chem., August 1, 2003; 278(31): 29048 - 29056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Johnson, R. C. Burghardt, M. M. Joyce, T. E. Spencer, F. W. Bazer, C. A. Gray, and C. Pfarrer Osteopontin Is Synthesized by Uterine Glands and a 45-kDa Cleavage Fragment Is Localized at the Uterine-Placental Interface Throughout Ovine Pregnancy Biol Reprod, July 1, 2003; 69(1): 92 - 98. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.B.C. Apparao, M. J. Illera, S. A. Beyler, G. E. Olson, K. G. Osteen, M. H. Corjay, K. Boggess, and B. A. Lessey Regulated Expression of Osteopontin in the Peri-Implantation Rabbit Uterus Biol Reprod, May 1, 2003; 68(5): 1484 - 1490. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang, Y.-G. Zhao, Y.-J. Cao, Q.-X. A. Sang, and E.-K. Duan Expression and implications of tissue inhibitor of metalloproteinases-4 in mouse embryo Mol. Hum. Reprod., March 1, 2003; 9(3): 143 - 149. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Inagaki, C. Stern, J. McBain, A. Lopata, L. Kornman, and D. Wilkinson Analysis of intra-uterine cytokine concentration and matrix-metalloproteinase activity in women with recurrent failed embryo transfer Hum. Reprod., March 1, 2003; 18(3): 608 - 615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Illera, P.L. Lorenzo, Y.-t. Gui, S. A. Beyler, K.B.C. Apparao, and B. A. Lessey A Role for {alpha}v{beta}3 Integrin During Implantation in the Rabbit Model Biol Reprod, March 1, 2003; 68(3): 766 - 771. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. C. Ajonuma, L. N. Chan, E. H. Y. Ng, P. H. Chow, L. S. Kung, A. N. Y. Cheung, C. Briton-Jones, I. H. Lok, C. J. Haines, and H. C. Chan Characterization of epithelial cell culture from human hydrosalpinges and effects of its conditioned medium on embryo development and sperm motility Hum. Reprod., February 1, 2003; 18(2): 291 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Kabir-Salmani, S. Shiokawa, Y. Akimoto, H. Hasan-Nejad, K. Sakai, S. Nagamatsu, K. Sakai, Y. Nakamura, A. Hosseini, and M. Iwashita Characterization of Morphological and Cytoskeletal Changes in Trophoblast Cells Induced by Insulin-Like Growth Factor-I J. Clin. Endocrinol. Metab., December 1, 2002; 87(12): 5751 - 5759. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rosbottom, C. L. Scudamore, H. von der Mark, E. M. Thornton, S. H. Wright, and H. R. P. Miller TGF-{beta}1 Regulates Adhesion of Mucosal Mast Cell Homologues to Laminin-1 Through Expression of Integrin {alpha}7 J. Immunol., November 15, 2002; 169(10): 5689 - 5695. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Creus, J. Ordi, F. Fabregues, R. Casamitjana, B. Ferrer, E. Coll, J. A. Vanrell, and J. Balasch {alpha}v{beta}3 integrin expression and pinopod formation in normal and out-of-phase endometria of fertile and infertile women Hum. Reprod., September 1, 2002; 17(9): 2279 - 2286. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Basak, R. Dhar, and C. Das Steroids Modulate the Expression of {alpha}4 Integrin in Mouse Blastocysts and Uterus During Implantation Biol Reprod, June 1, 2002; 66(6): 1784 - 1789. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Strandell and A. Lindhard Why does hydrosalpinx reduce fertility?: The importance of hydrosalpinx fluid Hum. Reprod., May 1, 2002; 17(5): 1141 - 1145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. B. C. Apparao, M. J. Murray, M. A. Fritz, W. R. Meyer, A. F. Chambers, P. R. Truong, and B. A. Lessey Osteopontin and Its Receptor {alpha}v{beta}3 Integrin Are Coexpressed in the Human Endometrium during the Menstrual Cycle But Regulated Differentially J. Clin. Endocrinol. Metab., October 1, 2001; 86(10): 4991 - 5000. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Johnson, F. W. Bazer, L. A. Jaeger, H. Ka, J. E. Garlow, C. Pfarrer, T. E. Spencer, and R. C. Burghardt Muc-1, Integrin, and Osteopontin Expression During the Implantation Cascade in Sheep Biol Reprod, September 1, 2001; 65(3): 820 - 828. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. S. Smyth, E. D. Reis, H. Vaananen, W. Zhang, and B. S. Coller Variable protection of {beta}3-integrin-deficient mice from thrombosis initiated by different mechanisms Blood, August 15, 2001; 98(4): 1055 - 1062. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
