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Induction of Interleukin-1 Production in Murine Sertoli Cells by Interleukin-1¹

^a Department of Obstetrics and Gynecology, Soroka University Medical Center and Department of Microbiology and Immunology and Department of Pathology, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel ^b Christian-Albrechts-University of Kiel Medical School, Kiel, Germany

In the present study we examined the involvement of interleukin (IL)-1, -1ß, FSH, and lipopolysaccharide (LPS) in the regulation of IL-1 and -1ß production by Sertoli cells under in vitro conditions. Sertoli cell cultures from immature mice produced constitutively basal levels of intracellular IL-1. Stimulation of Sertoli cell cultures with LPS (5 µg/ml) resulted in a maximal production of intracellular IL-1 2 h after the stimulation. Thereafter, these levels decreased but remained significantly higher within 24 h after stimulation than those in control cultures. The effect of LPS on IL-1 production was dose dependent. FSH did not show any effect on intracellular IL-1 production by Sertoli cells. IL-1 could not be detected in supernatants of unstimulated or stimulated Sertoli cell cultures. Sertoli cell cultures stimulated with recombinant IL-1 induced optimal intracellular levels of IL-1 within 2 h of stimulation. These levels remained high 24 h after stimulation. However, stimulation of Sertoli cell cultures with IL-1ß induced a peak of IL-1 production 8 h after stimulation. These levels decreased 24 h after the stimulation but were still found to be significantly higher than those in control cultures. The addition of IL-1 receptor antagonist (IL-1ra) to Sertoli cell cultures did not significantly alter their capacity to produce IL-1. However, the stimulatory effects of recombinant IL-1 on IL-1 production by Sertoli cell cultures were reversed by the concomitant addition of recombinant IL-1ra.

No immunoreactive IL-1ß could be detected in lysates or conditioned media of immature murine Sertoli cells under any of the stimulatory conditions outlined.

Our results may suggest the involvement of physiological (IL-1) and pathophysiological factors (LPS) in the regulation of spermatogenesis and spermiogenesis processes and male fertility.

First decision: 1 October 1999.

¹ This work was supported by a grant (No. 4467) from the Ministry of Health, Jerusalem, Israel. During the course of this work D.Z. was the recipient of a Deutscher Akademischer Austauschdienst (DAAD) scholarship.

² Correspondence. FAX: 7 6400932; huleihel{at}bgumail.bgu.ac.il

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