Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction 62, 1329-1334 (2000)
© 2000 Society for the Study of Reproduction, Inc.

Article

Differential Splicing of Three Gonadotropin-Releasing Hormone Transcripts in the Ovary of Seabream (Sparus aurata)1

Massimo Nabissia, Laura Soverchiaa, Alberta M. Polzonetti-Magni2,a, and Hamid R. Habibib

a Dipartimento di Scienze Morfologiche e Biochimiche Comparate, University of Camerino, 62032 Camerino (MC), Italy b Department of Biological Sciences, University of Calgary, Calgary, Alberta T2N 1N4, Canada

Previous studies demonstrated the presence of high-affinity GnRH binding sites and compounds with GnRH-like activity in the ovary of seabream, Sparus aurata, providing evidence for the role of GnRH as a paracrine/autocrine regulator of ovarian function in this species. In the present study, the expression of three forms of GnRH (salmon, chicken-II, and seabream) genes in this marine teleost species was demonstrated for the first time. Moreover, there is evidence for differential splicing and intronic expression of cGnRH-II and sbGnRH. Treatment of seabream follicle-enclosed oocytes with salmon GnRH stimulated reinitiation of oocyte meiosis, whereas chicken GnRH-II treatment was without effect. Novel information was also provided about organization of cGnRH-II and seabream GnRH transcripts, confirming that GnRH gene organization is maintained through evolution, despite changes in the size and sequence of exons and introns.

First decision: 6 April 1999.

1 This study was supported by a grant to Prof. Alberta M. Polzonetti-Magni from the MURST (PRIN) Italy.

2 Correspondence: Alberta Maria Polzonetti-Magni, Dipartimento di Scienze Morfologiche e Biochimiche Comparate, University of Camerino, Via Camerini no 3, 62032 Camerino (MC), Italy. FAX: 39 0737 403217; alberta{at}camserv.unicam.it




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S. L. Gray, B. A. Adams, C. M. Warby, K. R. von Schalburg, and N. M. Sherwood
Transcription and Translation of the Salmon Gonadotropin-Releasing Hormone Genes in Brain and Gonads of Sexually Maturing Rainbow Trout (Oncorhynchus mykiss)
Biol Reprod, November 1, 2002; 67(5): 1621 - 1627.
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