HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gadd, T.S. Articles by Wathes, D.C. Search for Related Content PubMed PubMed Citation Articles by Gadd, T.S. Articles by Wathes, D.C. Agricola Articles by Gadd, T.S. Articles by Wathes, D.C.

Regulation and Localization of Insulin-Like Growth Factor Binding Protein-5 Gene Expression in the Uterus and Placenta of the Cyclic and Early Pregnant Ewe¹

^a Department of Veterinary Basic Sciences, Royal Veterinary College, Potters Bar, Hertfordshire, EN6 1NB, United Kingdom

The insulin-like growth factor (IGF) system plays an important role in the regulation of uterine function and placental growth. However, there is little information regarding the localization and regulation of IGF binding protein-5 (IGFBP-5) in the reproductive tract. The distribution of this IGFBP was therefore investigated using in situ hybridization in sections of utero-placental tissue obtained throughout the estrous cycle, up to Day 55 of gestation, and on Days 16–17 from both horns of ewes with unilateral pregnancies that followed uterine transection. In nonpregnant ewes, IGFBP-5 mRNA was present at high concentrations in the maternal caruncles and luminal epithelium, and at moderate levels in myometrium. In these regions IGFBP-5 mRNA showed cyclic variations, with concentrations peaking around ovulation, whereas low expression in the endometrial stroma remained constant. During pregnancy, there was additional localization to the endometrial glands; and in all regions, with the exception of the caruncles, concentrations increased significantly with gestational age. In transected uteri, concentrations in the luminal epithelium of the pregnant horn were significantly higher than those in the nonpregnant horn. In the caruncles, IGFBP-5 mRNA formed an intense band just below the tips of the invading fetal villi. Below this band, IGFBP-5 mRNA localized to form a series of rings, which could create a route to allow the fetal villi access into the caruncular stroma for nutrient exchange. In conclusion, IGFBP-5 is abundantly expressed in the ovine reproductive tract, with both the concentration and localization differentially regulated during the cycle and pregnancy.

First decision: 10 September 1999.

¹ Financial support provided by the Wellcome Trust; J.C.O. was supported by a studentship from the Biotechnology and Biological Sciences Research Council.

² Correspondence: T.S. Gadd, Department of Veterinary Basic Sciences, The Royal Veterinary College, Boltons Park, Hawkshead Road, Potters Bar, Herts, EN6 1NB, United Kingdom. FAX: 01707 647085; treynold{at}rvc.ac.uk

This article has been cited by other articles:

				M. C. Satterfield, K. Hayashi, G. Song, S. G. Black, F. W. Bazer, and T. E. Spencer Progesterone Regulates FGF10, MET, IGFBP1, and IGFBP3 in the Endometrium of the Ovine Uterus Biol Reprod, December 1, 2008; 79(6): 1226 - 1236. [Abstract] [Full Text] [PDF]

				J. Kim, G. Song, H. Gao, J. L. Farmer, M. C. Satterfield, R. C. Burghardt, G. Wu, G. A. Johnson, T. E. Spencer, and F. W. Bazer Insulin-Like Growth Factor II Activates Phosphatidylinositol 3-Kinase-Protooncogenic Protein Kinase 1 and Mitogen-Activated Protein Kinase Cell Signaling Pathways, and Stimulates Migration of Ovine Trophectoderm Cells Endocrinology, June 1, 2008; 149(6): 3085 - 3094. [Abstract] [Full Text] [PDF]

				O. Shynlova, P. Tsui, A. Dorogin, B. L. Langille, and S. J. Lye Insulin-like Growth Factors and Their Binding Proteins Define Specific Phases of Myometrial Differentiation During Pregnancy in the Rat Biol Reprod, April 1, 2007; 76(4): 571 - 578. [Abstract] [Full Text] [PDF]

				C K Hilleson-Gayne and J A Clapper Effects of decreased estradiol-17{beta} on the serum and anterior pituitary IGF-I system in pigs J. Endocrinol., December 1, 2005; 187(3): 369 - 378. [Abstract] [Full Text] [PDF]

				M Munoz-Gutierrez, P A Findlay, C L Adam, G Wax, B K Campbell, N R Kendall, M Khalid, M Forsberg, and R J Scaramuzzi The ovarian expression of mRNAs for aromatase, IGF-I receptor, IGF-binding protein-2, -4 and -5, leptin and leptin receptor in cycling ewes after three days of leptin infusion Reproduction, December 1, 2005; 130(6): 869 - 881. [Abstract] [Full Text] [PDF]

				K. Hayashi, K. D Carpenter, T. H Welsh Jr, R. C Burghardt, L. J Spicer, and T. E Spencer The IGF system in the neonatal ovine uterus Reproduction, March 1, 2005; 129(3): 337 - 347. [Abstract] [Full Text] [PDF]