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a Department of Pediatrics, Harbor-UCLA Medical Center, Torrance, California 90509
b Howard Hughes Medical Institute, Departments of Biochemistry, Pediatrics and Genetics, Emory University School of Medicine, Atlanta, Georgia 30322
c Unidad de Investigacion, Hospital Universitario de Canarias and Department of Pathology, Universidad de La Laguna, E-38071 Tenerife, Spain
The DAZ (Deleted in AZoospermia) gene family was isolated from a region of the human Y chromosome long arm that is deleted in about 10% of infertile men with idiopathic azoospermia. DAZ and an autosomal DAZ-like gene, DAZL1, are expressed in germ cells only. They encode proteins with an RNA recognition motif and with either a single copy (in DAZL1) or multiple copies (in DAZ) of a DAZ repeat. A role for DAZL1 and DAZ in spermatogenesis is supported by their homology to a Drosophila male infertility protein Boule and by sterility of Dazl1 knock-out mice. The biological function of these proteins remains unknown. We found that DAZL1 and DAZ bound similarly to various RNA homopolymers in vitro. We also used an antibody against the human DAZL1 to determine the subcellular localization of DAZL1 in mouse testis. The sedimentation profiles of DAZL1 in sucrose gradients indicate that DAZL1 is associated with polyribosomes, and further capture of DAZL1 on oligo(dT) beads demonstrates that the association is mediated through the binding of DAZL1 to poly(A) RNA. Our results suggest that DAZL1 is involved in germ-cell specific regulation of mRNA translation.
1 This work was supported by NIH grants HD28009 and HD36347 (P.H.Y.). S.T.W. is an Investigator of the Howard Hughes Medical Institute.
2 Correspondence: Pauline Yen, Division of Medical Genetics, Harbor-UCLA Medical Center, 1124 W. Carson Street, Torrance, CA 90502-2064. FAX: 310 328 9921; pyen{at}rei.edu
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