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Cell Type-Specific Regulation of Fetal Fibronectin Expression in Amnion: Conservation of Glucocorticoid Responsiveness in Human and Nonhuman Primates¹

^a Department of Obstetrics and Gynecology, New York University School of Medicine, New York, New York 10016 ^b Department of Physiology, University of Cambridge, Cambridge, United Kingdom ^c Laboratory for Pregnancy and Newborn Research, Department of Physiology, Cornell University, Ithaca, New York 14853-6401

The appearance of oncofetal fibronectin (FFN) in cervical and vaginal secretions is predictive of human labor. Levels of FFN in amnion increase with the onset of labor in rhesus monkeys. Since glucocorticoid (GC) levels in serum and amniotic fluid increase in association with parturition, we compared GC-mediated regulation of FFN expression in cultures of amnion epithelial cells and fibroblasts isolated from human and baboon amnions. Cells were maintained with and without dexamethasone (DEX), and levels of FFN in the conditioned media were determined by ELISA. We observed that DEX treatment suppressed FFN levels in both human and baboon amnion epithelial cells, whereas it increased FFN levels in amnion fibroblasts. DEX treatment reduced FFN levels in cytotrophoblasts from human placenta and increased FFN levels in placental fibroblasts. Northern blots revealed that DEX reduced levels of fibronectin (FN) mRNA in amnion epithelial cells and cytotrophoblasts, whereas it increased FN mRNA in amnion and placental fibroblasts. We conclude that GC differentially regulates FFN expression in epithelial and mesenchymal cells from amnion and placenta. In addition, this pattern of cell type-specific FFN regulation by GC is conserved in human and nonhuman primates and may be responsible for parturition-dependent changes in FFN expression in gestational tissues.

First decision: 20 October 1999.

¹ These studies were supported in part through NIH grant HD 33909 (S.G.) and HD 21350 (P.W.N.).

² Correspondence: Seth Guller, Dept. OB/GYN, NYU School of Medicine, 550 First Ave., New York, NY 10016. FAX: 212 263 5742; gulles01{at}mcrcr.med.nyu.edu

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