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Somatostatin Inhibits Follicle-Stimulating Hormone-Induced Adenylyl Cyclase Activity and Proliferation in Immature Porcine Sertoli Cell via sst2 Receptor¹

^a Institut National de la Santé et de la Recherche Médicale (INSERM) Unité 407, Faculté de Médecine Lyon Sud, F;ch69921 Oullins, France

ABSTRACT

The potential involvement of somatostatin (SRIF) in testicular function was studied by using as a model primary cultures of purified immature porcine Sertoli cells. In the present report we show that Sertoli cells express mRNA for sst2 SRIF receptor and display SRIF-sensitive adenylyl cyclase. Sensitivity of adenylyl cyclase to SRIF and its analogues is compatible with the pharmacological profile of this receptor type. Relevant cAMP production is similarly inhibited by SRIF in both basal and stimulated (by gonadotropin FSH or by forskolin) conditions. Moreover, the observed SRIF actions on Sertoli cells require functional coupling of specific membrane receptors to adenylyl cyclase via Gi proteins because pertussis toxin prevents SRIF-dependent inhibition of adenylyl cyclase in either basal or FSH-stimulated conditions. Given the potent antiproliferative actions of SRIF in other cell types, we further assessed the possible SRIF-dependent modulation of [³H]thymidine incorporation by Sertoli cells. Our data point to SRIF-mediated inhibition of both basal and FSH-stimulated [³H]thymidine uptake. This inhibition of Sertoli cell proliferation is, at least in basal conditions, also blocked by pertussis toxin pretreatment. Altogether, these data suggest that SRIF may play a role as an (local) inhibitor of FSH actions in testicular development.

FOOTNOTES

First decision: 7 January 2000.

¹ Supported by INSERM (Unité 407), ARC grant 7244 (S.K.) and Ligue contre le Cancer, Comité de l'Ardèche (S.K.).

² Correspondence: Slavica Krantic, Laboratoire de Communication cellulaire en Biologie de la reproduction, INSERM 407, Faculté de Médecine Lyon Sud, B.P. 12, F-69921 Oullins, Cedex, France. FAX: 33 4 78 86 31 16; krantic{at}lyon151.inserm.fr

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