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Regular Article |
a Leland J. and Dorothy H. Olson Center for Women's Health, Departments of Ob/Gyn and Physiology and Biophysics, University of Nebraska Medical Center, Omaha, Nebraska 68198-4515
ABSTRACT
The hormonal regulation of ovarian transforming growth factor-ß (TGF-ß) type I receptor (TßRI) and TßRII messenger (mRNA) expression was evaluated using cyclic and hypophysectomized hamsters. Northern blot analysis revealed that three TßRI and one TßRII gene transcripts were expressed in the hamster ovary. Reverse transcription-polymerase chain reaction quantitation revealed that receptor mRNA was differentially expressed during the estrous cycle. Although, mRNA levels for both receptor types increased steadily up to Day 4:0900 h, a sharp decline occurred following the gonadotropin surge. In fact, receptor mRNA started declining by Day 4:1200 h, long before the gonadotropin surge; however, only TßRI mRNA levels recovered partially by 1500 h to fall again by 1600 h. Although hypophysectomy preferentially reduced TßRII mRNA levels, gonadotropins as well as ovarian steroids significantly induced TßRI and TßRII mRNA expression within 48 h and 24 h, respectively; 5
-dihydrotesterone (DHT) induced only TßRII mRNA. The induction of ovarian TßRI and TßRII mRNA by estradiol-17ß or progesterone was severely attenuated by dexamethasone. A marked increase in serum cortisol coincided with the periovulatory rise in serum gonadotropins. These results suggest that the increase in TGF-ß receptor mRNA expression correlates with gonadotropin-induced ovarian follicular development during the estrous cycle. Moreover, receptor mRNA expression is critically and differentially regulated by gonadotropins as well as ovarian steroids. Most importantly, glucocorticoid appears to play a critical modulatory role in the temporal expression of receptor mRNA in the ovary, hence, controlling folliculogenesis.
First decision: 29 December 1999.
1 This study was supported by a grant (HD28165) from NICHHD and the Olson Foundation of Omaha.
2 Correspondence: S.K. Roy, Department of Ob/Gyn and Physiology and Biophysics, BH4030, University of Nebraska Medical Center, 984515 Nebraska Medical Center, Omaha, NE 68198-4515. FAX: 402 559 6164; skroy{at}unmc.edu
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