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Production and Regulation of Cytokine-Induced Neutrophil Chemoattractant in Rat Ovulation

^a Department of Obstetrics and Gynecology, University of Tokushima School of Medicine, Tokushima 770-8503, Japan

ABSTRACT

A cytokine-induced neutrophil chemoattractant (CINC/gro), which belongs to the interleukin (IL)-8 family, acts as a functional chemoattractant for neutrophils in rats. In the present study, we examined whether CINC/gro contributes to the ovulation process in the rat ovulation system. In rat ovaries, CINC/gro was immunohistochemically recognized in the theca layer of the antral follicle but not in the granulosa cells. To clarify the role of CINC/gro in the ovulation process, CINC/gro protein and mRNA were examined during pregnant mare serum gonadotropin (PMSG)-hCG treatment. CINC/gro protein did not increase as a result of PMSG injection. However, it increased rapidly after hCG injection and peaked at 6 h after hCG. CINC/gro mRNA was also strongly expressed after hCG injection. The increase of CINC/gro protein followed increases in IL-1ß and tumor necrosis factor (TNF). In the whole ovarian dispersate culture, FSH, hCG, IL-1ß, and TNF stimulated the production of CINC/gro protein in a dose-dependent manner. In particular, the stimulatory effects of IL-1ß and TNF were stronger than those of gonadotropins. These results suggest that CINC/gro plays an important role in the rat ovulation process by attracting neutrophils. CINC/gro increased just prior to ovulation, and it may be regulated directly by cytokines such as IL-1ß and TNF and indirectly by gonadotropins.

FOOTNOTES

First decision: 3 December 1999.

¹ Correspondence: Kenjiro Ushigoe, Department of Obstetrics and Gynecology, University of Tokushima School of Medicine, 3-18-15, Kuramoto-cho, Tokushima 770-8503, Japan. FAX: 81 88 631 2630; ushi{at}clin.med.tokushima-u.ac.jp

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