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Regular Article |
a Bekesy Laboratory of Neurobiology, University of Hawaii at Manoa, Honolulu, Hawaii 96822
b Department of Anatomy and Reproductive Biology, John A. Burns School of Medicine, University of Hawaii at Manoa, Honolulu, Hawaii 96822
ABSTRACT
Since the first successful cloning of mammals from adult somatic cells, there has been no examination of the learning or behavior of cloned offspring. The possibility of adverse effects on animals produced through adult somatic cell cloning is high because many natural biological processes are bypassed and DNA from adult cells, which presumably contain mutations, are used. In this study, we compared cloned mice produced by microinjection transfer of cumulus cell nuclei into enucleated oocytes, to control mice that were specifically generated to eliminate confounding factors that are unique to our cloning procedure. Postnatal weight gain of clones was significantly greater than that of controls. Preweaning development observations revealed that first appearance or performance of 3 out of 10 measures was delayed in cloned mice; however, results of subsequent tests of learning and memory, activity level, and motor skills were comparable for both groups. Together, these data suggest that nuclear transfer of adult somatic cell nuclei to produce cloned mice may delay the appearance of a few developmental milestones but it does not adversely affect the overall postnatal behavior of mice. In addition, this procedure may cause late onset of significantly increased body weight in cloned offspring, the cause or causes of which are being further examined.
First decision: 4 January 2000.
1 Supported by funds from the Victoria S. and Bradley L. Geist Foundation, the Kosasa Family Foundation, and Pro-Bio America, Inc.
2 Correspondence: R. Yanagimachi, Department of Anatomy and Reproductive Biology, University of Hawaii John A. Burns School of Medicine, 1960 East-West Road, Honolulu, HI 96822. FAX: 808 956 5474; yana{at}hawaii.edu
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